国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2014, Vol. 33 ›› Issue (5): 339-341.

• 论著 • 上一篇    下一篇

盆炎消灌肠液改善小鼠微循环障碍的实验研究

付正英，鲍鳗夕，陈宁   

  1. 300162 天津，武警后勤学院附属医院（付正英，陈宁）；武警海南总队医院（鲍鳗夕）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-09-15 发布日期:2014-09-15
  • 通讯作者: 付正英

Experimental Study of Pen Yan Xiao Enema Improving Microcirculation in Mice

FU Zheng-ying，BAO Man-xi，CHEN Ning   

  1. Affiliated of Logistic University of Chinese People′s Armed Police Force，Tianjin 300162，China（FU Zheng-ying，CHEN Ning）；Police Armed Corps Hospital of Hainan，Haikou 570203，China（BAO Man-xi）
  • Received:1900-01-01 Revised:1900-01-01 Published:2014-09-15 Online:2014-09-15
  • Contact: FU Zheng-ying

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摘要： 目的：探讨盆炎消灌肠液对小鼠微循环障碍的改善作用。方法：选用小鼠60只，随机分为对照组、模型组、盆炎消灌肠液（2.5，5.0，10.0 g/kg）3个剂量组和盐酸川芎嗪组共6组，每组10只。采用高分子右旋糖酐建立小鼠微循环障碍模型。应用WX-9型微循环显微镜和微循环检测分析系统，观察小鼠微循环变化，观察在给药前及给药后5，10，20，30 min不同时间点，毛细血管管径大小、血液流速、血液流态及红细胞聚集性等指标的变化。结果：盆炎消灌肠液3个剂量组在给药后不同时间点均能改善微循环障碍，增大毛细血管管径，加快血液流速，改善血液流态，降低红细胞聚集性。随着剂量增加和给药时间的延长，改善微循环障碍的作用更加明显，效果更好。大剂量（10.0 g/kg）组与模型组比较差异有统计学意义（P＜0.05）。结论：盆炎消灌肠液能明显对抗由高分子右旋糖酐所致小鼠微循环障碍，改善盆腔微循环。

关键词: 灌肠, 微循环, 动物实验, 模型, 动物

Abstract: Objective: To explore the improved effect of the Pen Yan Xiao enema on microcirculation in mice. Methods：Sixty adult mice were used. Ten mice were used as control group. Fifty mice were induced with the high-molecular dextran to develop animal models of microcirculation disturbance, and then randomly divided into 5 groups as follows, model group, three groups treated with Pen Yan Xiao enema liquid（2.5, 5.0, 10.0 g/kg）, and a group treated with Chuanxiongzine as positive control. Microcirculation in mice was compared before treatment and 5, 10, 20, 30 min after treatment using the WX-9 microcirculation microscope and the microcirculation detection and analysis system. Those parameters were analyzed, including the diameter size of capillary, the blood flow velocity, the blood flow pattern and the red cell aggregation. Results：Pen Yan Xiao enema can improve microcirculation in the model mice in a dose-dependent manner and a time-dependent manner. In three treatment groups, the diameter size of capillary and the blood flow velocity were increased; the blood flow pattern was improved, while the red cell aggregation was decreased. Those effects of large doses（10.0 g/kg） of Pen Yan Xiao enema were significant（P＜0.05）. Conclusions：Pen Yan Xiao enema can significantly improve microcirculation in the animal model of microcirculation disturbance induced by high polymer dextran, suggesting the pharmacologic action of Pen Yan Xiao on microcirculation.

Key words: Enema, Microcirculation, Animal experimentation, Models, animal