国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2017, Vol. 36 ›› Issue (2): 93-96.

• 论著 •    下一篇

雌激素干预对去卵巢大鼠骨组织的影响

魏双双1,张治芬1,黄哲人2,何轶然1,吴红艳1,刘文华3,汤珊珊3,黄坚3   

  1. 1. 南京医科大学附属杭州医院
    2. 杭州市第一人民医院(南京医科大学附属杭州医院)
    3. 杭州市妇产科医院
  • 收稿日期:2017-01-16 修回日期:2017-02-11 出版日期:2017-03-15 发布日期:2017-03-15
  • 通讯作者: 张治芬 E-mail:zhangzf@zju.edu.cn
  • 基金资助:
    国家卫生计生委科学研究基金-浙江省医药卫生重大科技计划;杭州市科技局重点项目;杭州市科技局重点项目;杭州市医药卫生科技计划重点项目;浙江省科技厅重大科技专项重点项目

Effects of estrogen on osteoporosis in ovariectomized rats.

  • Received:2017-01-16 Revised:2017-02-11 Published:2017-03-15 Online:2017-03-15

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摘要： 【摘要】目的 建立绝经后骨质疏松模型，外源性补充雌激素，探讨NFATcl在去卵巢大鼠骨丢失中的作用，以及雌激素的骨保护作用。方法 3月龄Wistar雌性大鼠随机分为Sham组、OVX组、OVX+E2组，每组10只。OVX+E2组给予17β-E2皮下注射，其余2组给予等量生理盐水。16周后测定骨组织中NFATcl表达量，Micro-CT分析大鼠胫骨平台处骨密度（BMD）、骨体积分数（BV/TV）、骨小梁厚度（Tb.Th）、骨小梁数目（Tb.N）、骨小梁间距（Tb.SP）变化，ELISA法测定血清 E2水平。结果 Sham组、OVX+E2组与OVX组相比，Tb.Th、Tb.N、BV/TV、血清E2水平明显升高，NFATcl、Tb.Sp水平明显下降(均P<0.05)。结论 雌激素可能通过抑制NFATcl的表达减缓骨量丢失。

关键词: 雌激素, NFATcl, 绝经后骨质疏松

Abstract: 【Abstract】Objectives To investigate mechanism of NFATcl in bone loss in a pastmenopausal osteoporosis(PMO)rat mode with ovarletomy(OVX) , and to explore the protective effects of exogenous estrogen in bone tissue. Methods The three months Wistar rats were randomly divided into sham operation group, OVX group, and OVX+E2 group and 10 rats in each group. In the OVX+E2 group, 17β-E2 was injected subcutaneously and the other two groups were given the same volume of normal saline. The of NFATcl in bone tissue were measured after 16 weeks. The bone mineral density (BMD), the fraction of bone tissues(BV/IV), The thickness of the trabecular bone (Tb.Th), number of trabecular bone (Tb.N) and thetrabecular spacing (Tb.SP) were measuredusing Micro-CT analysis, and serum E2 level was determined by ELISA. Results The OVX+E2 group and sham operation group compared with the OVX group, the levels of BMD, BV/TV, Tb.Th, Tb.N and serum E2 were significantly increased, the level of Tb.SP and NFATcl were significantly decreased. Conclusion Estrogen may prevent bone loss by decreasing NFATcl in OVX rats．

Key words: Estrogen, NFATcl, osteoporosis.