国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2017, Vol. 36 ›› Issue (3): 251-255.

• 论著 • 上一篇    下一篇

早期胚胎停育绒毛组织的蛋白质组学分析

姚婷，刘国忠，侯海燕，秦喆，陈娟，刘健，陈亚琼   

  1. 300193 天津中医药大学（姚婷）；中国人民武装警察部队后勤学院附属医院妇产科（姚婷，刘国忠，侯海燕，秦喆，陈娟，刘健，陈亚琼）；中国医学科学院北京协和医学院（侯海燕）
  • 收稿日期:2017-02-21 修回日期:2017-03-14 出版日期:2017-05-15 发布日期:2017-05-15
  • 通讯作者: 陈亚琼，E-mail：chenyq82@hotmail.com E-mail:yaotingllw@163.com
  • 基金资助:
    国家自然科学基金（81402691）；天津市应用基础与前沿技术研究计划（15JCZDJC36000，15JCQNJC12300）

Proteomic Analysis on Villous Tissues Derived from Pregnant Women of Early Embryo Damage

YAO Ting, LIU Guo-zhong, HOU Hai-yan, QIN Zhe, CHEN Juan, LIU Jian, CHEN Ya-qiong   

  1. Tianjin University of Tranditional Chinese Medicine，Tianjin 300193, China（YAO Ting）；Department of Obstetrics and Gynecology, Affiliated Hospital of Medical College of Chinese People′s Armed Police Forces, Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazards, Tianjin 300162, China（YAO Ting, LIU Guo-zhong, HOU Hai-yan, QIN Zhe, CHEN Juan, LIU Jian, CHEN Ya-qiong）；Chinese Academy and Medical Sciences & Peking Union Medical College, Beijing 100730, China（HOU Hai-yan）
  • Received:2017-02-21 Revised:2017-03-14 Published:2017-05-15 Online:2017-05-15
  • Contact: CHEN Ya-qiong, E-mail:chenyq82@hotmail.com E-mail:yaotingllw@163.com

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摘要： 目的：应用蛋白质组学方法研究胎儿染色体正常早期胚胎停育患者与胎儿染色体正常且正常妊娠行人工流产患者绒毛组织蛋白表达情况，寻找早期胚胎停育发病机制的可能线索。方法：选取10例胎儿染色体正常早期胚胎停育患者及5例胎儿染色体正常且正常妊娠行人工流产患者为研究对象，应用蛋白质组学高效液相色谱/串联质谱联用技术（LC-MS/MS）检测绒毛组织蛋白质谱，应用Maxquant软件、Uniprot人类参考蛋白质数据库、DAVID Analysis数据库分析质谱数据，找出并分析差异蛋白。结果：质谱共鉴定3 950个蛋白，定量3 879个蛋白，早期胚胎停育患者与对照组比较共筛选出220个差异蛋白，其中162个蛋白上调，58个蛋白下调，这些差异蛋白主要参与免疫反应、活性氧代谢、血管生成等生物过程，另外C1R、C3、C4A、C4B、C7表达上调，与补体系统激活有关；TPSO表达上调，与活性氧代谢有关。结论：建立了绒毛组织中早期胚胎停育相关的差异表达蛋白质谱。胎盘组织补体系统的过度激活及氧化还原反应失衡可能参与了早期胚胎停育的发病机制。

关键词: 早期胚胎停育, 蛋白质组学, 补体激活, 活性氧, 氧化还原

Abstract: Objective: The differentially expressed proteins in the villous tissues derived from those women of fetal normal chromosome carly embryo damage(EED) and those women asked abortion with non-medical reasons were analyzed with the proteomic method, so as to search some clues of the pathogenesis of EED. Methods: Ten EED patients with fetal normal chromosome (the EED group) and 5 women who asked abortion with non-medical reasons (the control group) were included in this study. The LC-MS/MS, proteomic technology, was use to detect the protein map of villous tissues. The Maxquant software, Uniprot human reference protein database and DAVID Analysis were used to analyse the differential expression profile of proteins. Results: MS experiments identified 3 950 proteins, and qualified 3 879 proteins. A total of 220 proteins were found to be differently expressed including 162 up-regulated proteins and 58 down-regulated proteins in that profile. These proteins were involved in several biological process, such as immune response, reactive oxygen species metabolic process, angiogenesis and so on. The up-regulated C1R, C3, C4A, C4B and C7 were invoved in the activation of complement system, and the up-regulated TPSO was involved in the metabolism of reactive oxygen species. Conclusions: The differential expression profile of proteins was developed. The excessive activation of complement system, and the imbalance of redox reaction, could be related with the pathogenesis of EED.

Key words: Early embryo damage, Proteomics, Complement activation, Reactive oxygen species, Oxidation-reduction