国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2016, Vol. 35 ›› Issue (1): 17-20.

• 论著 • 上一篇    下一篇

极性蛋白Par3在小鼠胚胎着床子宫内膜中的表达

陈明明，徐望明，肖卓妮，解美婷   

  1. 430060 武汉大学人民医院妇产科
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2016-01-15 发布日期:2016-01-15
  • 通讯作者: 徐望明

Expression of Polarity Protein Par3 in Mouse Endometrium during Embryo Implantation

CHEN Ming-ming，XU Wang-ming，XIAO Zhuo-ni，XIE Mei-ting   

  1. Department of Obstetrics and Gynecology，Renmin Hospital of Wuhan University，Wuhan 430060，China.
  • Received:1900-01-01 Revised:1900-01-01 Published:2016-01-15 Online:2016-01-15
  • Contact: XU Wang-ming

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摘要： 目的：探讨极性蛋白Par3在不同小鼠模型子宫内膜中的表达水平及其在小鼠胚胎着床过程中的作用。方法：建造妊娠1～8 d（早孕组）、假孕1～5 d（假孕组）、卵巢切除后类固醇激素注射（类固醇激素处理组）小鼠模型，应用免疫组织化学方法检测各种模型中小鼠子宫内膜腔上皮中Par3的表达情况，并使用Image-Pro Plus（IPP）图像分析系统进行半定量分析。结果：早孕组妊娠1～4 d小鼠内膜腔上皮中持续表达Par3并于妊娠4 d达高峰，于妊娠5 d明显减弱，妊娠6～8 d围绕胚胎初级蜕膜区域阴性表达并于次级蜕膜区域阳性表达；假孕组Par3表达趋势与早孕组类似，趋势变化幅度较早孕组小；类固醇激素处理组中，注射雌二醇（E2）组、孕酮（P4）组以及同时注射E2+P4组的Par3表达水平均明显低于注射芝麻油对照组。结论：雌、孕激素可下调Par3表达，Par3表达水平下降引起子宫内膜腔上皮极性短暂下调有利于胚胎着床。

关键词: 胚胎植入, 子宫内膜, 细胞极性, GTP结合蛋白质类, 小鼠

Abstract: Objective：To analyse the expression of polarity protein Par3, and its possible effects, in the mouse endometrium during embryo implantation. Methods：The mice models of early pregnancy （1-8 days of pregnancy）, pseudo-pregnancy and the steroid treatment （steroid injection in the emasculated mice） were used in this study. The expression of Par3 in uterus were tested by immunohistochemistry and analyzed by Image-Pro Plus system. Results： Expression of Par3 was increased in the uterine luminal epithelium on 1 to 4 days of early pregnancy, and then lowered on the day 5 of pregnancy. Interestingly, the positive expression of Par3 was found in the secondary decidual zone on the day 6 to 8 of pregnancy, while the negative expression in the primary decidual zone. Pseudo-pregnant models have a similar expression pattern as the early pregnant models, with low expression levels. In ovariectomized mice, both E2 and P4 treatment, or the combined treatment, could down-regulate the expression of Par3 in the luminal epithelium. Conclusions： The expression of Par3 in the uterine luminal epithelium of early pregnancy can be briefly down-regulated by E2 and P4, which is benefit for embryo implantation.

Key words: Embryo implantation, Endometrium, Cell polarity, GTP-binding proteins, Mice