环状RNA在子宫内膜异位症不孕患者卵泡颗粒细胞中的作用

doi:10.12280/gjszjk.20230529

摘要/Abstract

摘要：

卵泡发育异常是子宫内膜异位症（endometriosis，EMs）合并不孕的病理机制之一。近年，环状RNA（circular RNA，circRNA）在子宫内膜相关疾病发生过程中的作用日益引起关注，但是circRNA在EMs中的研究尚处于起步阶段，尤其是circRNA在EMs卵泡发育中的作用仍不明确。目前已知circRNA能够通过影响卵泡颗粒细胞增殖与凋亡、上皮-间质转化以及卵泡液微环境和激素调节，参与EMs的发生发展过程，并进一步导致不孕。阐述circRNA可能影响卵泡颗粒细胞从而导致不良妊娠结局的研究进展，探讨circRNA作为EMs生物标志物的可能性，以期为临床提供诊治的新思路和新方法。

关键词: 子宫内膜异位症, 不育, 女（雌）性, RNA, 环状, 卵泡, 粒层细胞

Abstract:

The abnormal follicular development is one of the pathological mechanisms of infertility in patients with endometriosis (EMs). In recent years, the role of circular RNA (circRNA) in the development of endometrium-related diseases has attracted more attention. However, the research on the role of circRNAs in EMs is still at the early stage, and the role of circRNA in the development of EMs follicles is especially unclear. It is currently known that circRNAs can participate in the occurrence and development of EMs by affecting the proliferation and apoptosis of granulosa cells, epithelial-mesenchymal transition, microenvironment of follicular fluid and hormone regulation, which further lead to infertility. This review aims to elucidate the research progress on the potential impact of circRNAs on follicular granulosa cells leading to adverse pregnancy outcomes, and to explore the possibility of circRNAs as a biomarker for EMs, so as to provide new ideas and methods for clinical diagnosis and treatment.

Key words: Endometriosis, Infertility, female, RNA, circular, Ovarian follicle, Granulosa cells

高朝阳, 章宁晴, 陈琼华, 吴荣锋. 环状RNA在子宫内膜异位症不孕患者卵泡颗粒细胞中的作用[J]. 国际生殖健康/计划生育杂志, 2024, 43(3): 243-248.

GAO Zhao-yang, ZHANG Ning-qing, CHEN Qiong-hua, WU Rong-feng. The Role of CircRNAs in Follicular Granulosa Cells of Patients with Endometriosis Infertility[J]. Journal of International Reproductive Health/Family Planning, 2024, 43(3): 243-248.

参考文献 33

[1]	Ma Y, Xu Y, Zhang J, et al. Biogenesis and functions of circular RNAs and their role in diseases of the female reproductive system[J]. Reprod Biol Endocrinol, 2020, 18(1):104. doi: 10.1186/s12958-020-00653-5.
[2]	Hayashi S, Nakamura T, Motooka Y, et al. Novel ovarian endometriosis model causes infertility via iron-mediated oxidative stress in mice[J]. Redox Biol, 2020, 37:101726. doi: 10.1016/j.redox.2020.101726.
[3]	Huang X, Yu Q. Bioinformatic analysis confirms differences in circular RNA expression profiles of cumulus cells between patients with ovarian and peritoneal endometriosis-associated infertility[J]. Front Endocrinol(Lausanne), 2023, 14:1137235. doi: 10.3389/fendo.2023.1137235.
[4]	Misir S, Wu N, Yang BB. Specific expression and functions of circular RNAs[J]. Cell Death Differ, 2022, 29(3):481-491. doi: 10.1038/s41418-022-00948-7. pmid: 35169296
[5]	Sanger HL, Klotz G, Riesner D, et al. Viroids are single-stranded covalently closed circular RNA molecules existing as highly base-paired rod-like structures[J]. Proc Natl Acad Sci U S A, 1976, 73(11):3852-3856. doi: 10.1073/pnas.73.11.3852.
[6]	Jiao J, Duan C, Zheng J, et al. Development of a two-in-one integrated assay for the analysis of circRNA-microRNA interactions[J]. Biosens Bioelectron, 2021, 178:113032. doi: 10.1016/j.bios.2021.113032.
[7]	Wu H, Pan X, Yang Y, et al. Recognizing binding sites of poorly characterized RNA-binding proteins on circular RNAs using attention Siamese network[J]. Brief Bioinform, 2021, 22(6):bbab279. doi: 10.1093/bib/bbab279.
[8]	Liu CX, Chen LL. Circular RNAs: Characterization, cellular roles, and applications[J]. Cell, 2022, 185(12):2016-2034. doi: 10.1016/j.cell.2022.04.021.
[9]	Pan X, Fang Y, Li X, et al. RBPsuite: RNA-protein binding sites prediction suite based on deep learning[J]. BMC Genomics, 2020, 21(1):884. doi: 10.1186/s12864-020-07291-6. pmid: 33297946
[10]	Lei M, Zheng G, Ning Q, et al. Translation and functional roles of circular RNAs in human cancer[J]. Mol Cancer, 2020, 19(1):30. doi: 10.1186/s12943-020-1135-7. pmid: 32059672
[11]	Shen HH, Zhang T, Yang HL, et al. Ovarian hormones-autophagy-immunity axis in menstruation and endometriosis[J]. Theranostics, 2021, 11(7):3512-3526. doi: 10.7150/thno.55241.
[12]	Wu R, Li J, Li J, et al. Circular RNA expression profiling and bioinformatic analysis of cumulus cells in endometriosis infertility patients[J]. Epigenomics, 2020, 12(23):2093-2108. doi: 10.2217/epi-2020-0291. pmid: 33090019
[13]	Hung SW, Zhang R, Tan Z, et al. Pharmaceuticals targeting signaling pathways of endometriosis as potential new medical treatment: A review[J]. Med Res Rev, 2021, 41(4):2489-2564. doi: 10.1002/med.21802. pmid: 33948974
[14]	Zhao H, Wang L, Wang Y. Circulating microRNAs as candidate biomarkers for the ovarian response during in vitro fertilization[J]. Medicine(Baltimore), 2021, 100(6):e24612. doi: 10.1097/MD.0000000000024612.
[15]	Kalous J, Aleshkina D, Anger M. A Role of PI3K/Akt Signaling in Oocyte Maturation and Early Embryo Development[J]. Cells, 2023, 12(14):1830. doi: 10.3390/cells12141830.
[16]	Hon JX, Wahab NA, Karim A, et al. MicroRNAs in Endometriosis: Insights into Inflammation and Progesterone Resistance[J]. Int J Mol Sci, 2023, 24(19):15001. doi: 10.3390/ijms241915001.
[17]	Meng L, Jan SZ, Hamer G, et al. Preantral follicular atresia occurs mainly through autophagy, while antral follicles degenerate mostly through apoptosis[J]. Biol Reprod, 2018, 99(4):853-863. doi: 10.1093/biolre/ioy116. pmid: 29767707
[18]	Cela V, Malacarne E, Obino M, et al. Exploring Epithelial-Mesenchymal Transition Signals in Endometriosis Diagnosis and In Vitro Fertilization Outcomes[J]. Biomedicines, 2021, 9(11):1681. doi: 10.3390/biomedicines9111681.
[19]	Yan H, Wen J, Zhang T, et al. Oocyte-derived E-cadherin acts as a multiple functional factor maintaining the primordial follicle pool in mice[J]. Cell Death Dis, 2019, 10(3):160. doi: 10.1038/s41419-018-1208-3. pmid: 30770786
[20]	Verón GL, Veiga MF, Cameo M, et al. Epithelial and Neural Cadherin in Mammalian Fertilization: Studies in the Mouse Model[J]. Cells, 2021, 11(1):102. doi: 10.3390/cells11010102.
[21]	Liu X, Wu J, Yang B, et al. hsa_circ_0005991 promotes epithelial-mesenchymal transition by regulating miR-30b-3p/Cdc42EP1 axis in ovary endometriosis[J]. Genomics, 2023, 115(6):110718. doi: 10.1016/j.ygeno.2023.110718.
[22]	He X, Liu N, Mu T, et al. Oestrogen induces epithelial-mesenchymal transition in endometriosis via circ_0004712/miR-148a-3p sponge function[J]. J Cell Mol Med, 2020, 24(17):9658-9666. doi: 10.1111/jcmm.15495.
[23]	Jia C, Wang S, Yin C, et al. Loss of hsa_circ_0118530 inhibits human granulosa-like tumor cell line KGN cell injury by sponging miR-136[J]. Gene, 2020, 744:144591. doi: 10.1016/j.gene.2020.144591.
[24]	Clower L, Fleshman T, Geldenhuys WJ, et al. Targeting Oxidative Stress Involved in Endometriosis and Its Pain[J]. Biomolecules, 2022, 12(8):1055. doi: 10.3390/biom12081055.
[25]	Li M, Ding W, Tariq MA, et al. A circular transcript of ncx1 gene mediates ischemic myocardial injury by targeting miR-133a-3p[J]. Theranostics, 2018, 8(21):5855-5869. doi: 10.7150/thno.27285.
[26]	Li Y, Liu H, Ye S, et al. The effects of coagulation factors on the risk of endometriosis: a Mendelian randomization study[J]. BMC Med, 2023, 21(1):195. doi: 10.1186/s12916-023-02881-z. pmid: 37226166
[27]	Bourdon M, Santulli P, Jeljeli M, et al. Immunological changes associated with adenomyosis: a systematic review[J]. Hum Reprod Update, 2021, 27(1):108-129. doi: 10.1093/humupd/dmaa038. pmid: 33099635
[28]	Cheng J, Huang J, Yuan S, et al. Circular RNA expression profiling of human granulosa cells during maternal aging reveals novel transcripts associated with assisted reproductive technology outcomes[J]. PLoS One, 2017, 12(6):e0177888. doi: 10.1371/journal.pone.0177888.
[29]	Saunders P, Horne AW. Endometriosis: Etiology, pathobiology, and therapeutic prospects[J]. Cell, 2021, 184(11):2807-2824. doi: 10.1016/j.cell.2021.04.041. pmid: 34048704
[30]	Suganuma I, Mori T, Ito F, et al. Peroxisome proliferator-activated receptor gamma, coactivator 1α enhances local estrogen biosynthesis by stimulating aromatase activity in endometriosis[J]. J Clin Endocrinol Metab, 2014, 99(7):E1191-E1198. doi: 10.1210/jc.2013-2525.
[31]	Yao T, Ying X, Zhao Y, et al. Vitamin D receptor activation protects against myocardial reperfusion injury through inhibition of apoptosis and modulation of autophagy[J]. Antioxid Redox Signal, 2015, 22(8):633-650. doi: 10.1089/ars.2014.5887.
[32]	Gong S, Sun GY, Zhang M, et al. Mechanisms for the species difference between mouse and pig oocytes in their sensitivity to glucorticoids[J]. Biol Reprod, 2017, 96(5):1019-1030. doi: 10.1093/biolre/iox026. pmid: 28419184
[33]	Zhang J, Wang C, Jia C, et al. The Role of Circular RNAs in the Physiology and Pathology of the Mammalian Ovary[J]. Int J Mol Sci, 2022, 23(23):15204. doi: 10.3390/ijms232315204.