N6-甲基腺嘌呤修饰在卵子发生及早期胚胎发育中的调控作用

doi:10.12280/gjszjk.20230089

摘要/Abstract

摘要：

N6-甲基腺嘌呤（N⁶-methyladenosine，m⁶A）是指RNA腺苷第6位氮（N）原子的甲基化修饰，是哺乳动物mRNA中最为丰富的表观转录组学修饰。m⁶A依赖于甲基转移酶（Writer）、去甲基化转移酶（Eraser）和m⁶A结合蛋白（Reader）的共同调控作用。诸多研究表明m⁶A及其调节酶几乎存在于各个发育阶段的卵泡及早期胚胎组织中，凭借其动态、可逆、敏感的特性广泛地参与mRNA的代谢过程，在转录后水平调控卵子发生，早期胚胎的核重编程、谱系分化、种植以及妊娠维持，在很大程度上决定了女性的生育能力和妊娠结局，并有望成为诸多生殖障碍相关疾病的诊断、预后标志物以及新的治疗靶点。

关键词: RNA，信使, 卵子发生, 胚胎发育, 甲基化

Abstract:

N⁶-methyladenosine (m⁶A) refers to the methylation modification at the N⁶ position of adenosine in RNA, which is the most abundant epitranscriptomic modification in mammalian mRNA. m⁶A depends on the co-regulatory effects of methyltransferase (Writer), demethylase (Eraser) and m⁶A binding protein (Reader). Numerous studies have shown that m⁶A and its regulatory enzymes are present in the follicles and early embryonic tissues at all development stages, and that m⁶A is extensively involved in the mRNA metabolic processes by virtue of their dynamic, reversible and sensitive properties. Therefore, m⁶A participates in the regulation of oogenesis, nuclear reprogramming, lineage differentiation, implantation, and gestation maintenance of early embryos at the post-transcriptional level. Because m⁶A is largely related to female fertility and pregnancy outcome, m⁶A may be a new marker of diagnosis and prognosis of many reproductive diseases, or a potential therapeutic target.

Key words: RNA, messenger, Oogenesis, Embryonic development, Methylation

闻鑫, 赵晓丽, 栾祖乾, 高娜, 董融, 夏天. N6-甲基腺嘌呤修饰在卵子发生及早期胚胎发育中的调控作用[J]. 国际生殖健康/计划生育杂志, 2023, 42(4): 310-316.

WEN Xin, ZHAO Xiao-li, LUAN Zu-qian, GAO Na, DONG Rong, XIA Tian. Regulatory Role of N⁶-Methyladenosine Modification in Oogenesis and Early Embryonic Development[J]. Journal of International Reproductive Health/Family Planning, 2023, 42(4): 310-316.

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