国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2012, Vol. 31 ›› Issue (4): 295-298.

• 综述 • 上一篇    下一篇

人类卵巢组织冷冻保存及其评价的研究进展

潘永苗, 徐向荣, 潘园园, 黄荷凤   

  1. 浙江大学医学院附属妇产科医院（潘园园现工作于温州医学院附属第二医院）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-07-15 发布日期:2012-07-15
  • 通讯作者: 黄荷凤

Cryopreservation of Human Ovarian Tissue and It's Evaluation

PAN Yong-miao，XU Xiang-rong， PAN Yuan-yuan， HUANG He-feng   

  1. Women's Hospital School of Medicine，Zhejiang University，Hangzhou 310006，China
  • Received:1900-01-01 Revised:1900-01-01 Published:2012-07-15 Online:2012-07-15
  • Contact: HUANG He-feng

PDF (PC)

6929

摘要： 恶性肿瘤及其治疗过程可能导致女性患者的卵巢功能衰竭，而卵巢组织冷冻保存使这些患者的生育储备以及生殖、内分泌功能的保存成为可能，具有重要的现实意义和广阔的应用前景。目前，卵巢组织冷冻保存技术包括慢速冷冻法、快速冷冻法和玻璃化冷冻法。卵巢组织冻融后主要通过组织学光镜观察卵母细胞和颗粒细胞形态；电镜下观察卵母细胞和间质细胞的线粒体、内质网、核膜和核染色质等超微结构改变评价冻融的影响；冻融组织培养后主要通过观察各卵泡，包括卵泡的直径大小、颗粒细胞的形态和层数、以及卵泡腔和透明带的形成和培养液中激素的含量等评价组织生长发育潜能；通过组织移植技术，检测移植宿主内分泌功能或生殖和内分泌功能的恢复是评价冻存复苏成败的重要指标和目标。

关键词: 卵巢, 培养技术, 器官保存, 低温保存, 复温, 移植, 自体

Abstract: Malignancy itself and its radiotherapy or chemotherapy result in premature ovarian failure. The cryopreservation of ovarian tissue may restore fertility and endocrine function for those patients，which is an important clinical technique. Laboratory methods of ovarian cryopreservation include slow-rate freezing，ultra-rapid cooling and vitrification. The histological morphology of oocytes and granular cells was observed by optical microscope after freezing and thawing ovarian tissue. Effect of freezing and thawing procedures on the ultrastructure of oocytes and interstitial cells，including mitochondria，endoplasmic reticulum，karyotheca，chromatin and so on，was evaluated by electron microscope. The potentiality of growth and development of ovarian tissue after culture was evaluated by oocyte diameter，shape and amount of granular cells，formation of follicular cavity and zona pellucida，hormonal levels in culture medium. It is the major index and goal to detect endocrinal function and reproductive recovery after tissue transplantation with the cryopreservated ovarian tissue.

Key words: Ovary, Culture techniques, Organ preservation, Cryopreservation, Rewarming, Transplan-tation, autologous