国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2014, Vol. 33 ›› Issue (6): 410-414.

• 论著 • 上一篇    下一篇

不同发育阶段大鼠睾丸中Calretinin的表达

刘珊，徐文丹，代晓南，王箐，罗建，高超，高莉，刘嘉茵，崔毓桂   

  1. 210029 南京医科大学第一附属医院生殖医学科，生殖医学国家重点实验室[刘珊（现工作于白求恩国际和平医院），徐文丹，代晓南，王箐，罗建，高超，高莉，刘嘉茵，崔毓桂]
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-11-15 发布日期:2014-11-15
  • 通讯作者: 崔毓桂

Expression of Calretinin in Testes of Rats at Different Development Stages

LIU Shan，XU Wen-dan, DAI Xiao-nan, WANG Jing, LUO Jian, GAO Chao, GAO Li, LIU Jia-yin, CUI Yu-gui   

  1. State Key Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China[LIU Shan（Now Working at Bethune International Peace Hospital），XU Wen-dan, DAI Xiao-nan, WANG Jing, LUO Jian, GAO Chao, GAO Li, LIU Jia-yin, CUI Yu-gui]
  • Received:1900-01-01 Revised:1900-01-01 Published:2014-11-15 Online:2014-11-15
  • Contact: CUI Yu-gui

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摘要： 目的：Calretinin（CALB2）是一种钙结合蛋白。前期研究表明，CALB2可表达于人睾丸组织，推测其参与睾丸功能的调节。本研究观察不同发育阶段大鼠睾丸中CALB2的表达水平，探讨其在调节雄激素生成中的作用。方法： 3～4周龄SD雄性大鼠作为性发育前组（相当于人类的青春期前，n=35），16周龄SD鼠作为性成熟组（相当于人类的成年期，n=16），12个月以上的作为老年组（相当于人类男子的中老年，n=10）。放射免疫法测定各组血清睾酮浓度；免疫组织化学法观察CALB2的定位表达；定量聚合酶链反应（qPCR）和蛋白质印迹法（Western Blot）分别检测睾丸中CALB2 mRNA和蛋白水平的表达。结果：CALB2表达定位于睾丸的间质细胞，细胞内定位于细胞质中。性发育前组大鼠血清睾酮水平最低，性成熟组最高，差异有统计学意义（P＜0.05）。性成熟组大鼠睾丸CALB2的表达水平较性发育前组和老年组均升高（P＜0.05）。结论：睾丸组织中，CALB2表达于间质细胞的细胞质；在不同发育阶段，睾丸间质细胞CALB2表达水平与血清睾酮水平呈正相关，表明CALB2参与调节雄激素生成。

关键词: 雄激素类, 睾丸, 莱迪希细胞, 钙结合蛋白, 维生素D依赖性

Abstract: Objective：Calretinin(CALB2), a calcium-binding protein, may be expressed in human testes, suggesting that CALB2 can play roles in regulating testicular functions. This study was designed to explore the different expression of CALB2 in the testes of rats at different development stags, and to discuss its role in regulating androgen production. Methods：The 3-4-week-old SD male rats as group A（the pre-sexual maturity, n=35），16-week-old as group B（the sexual maturity, n=16）， and over-12-month as group C（the older rats, n=10）were used in the study. Sera testosterone concentration was detected by radioimmunoassay（ＲＩＡ）.Cellular location of CALB2 in testes was observed by immunohistochemistry. Expression levels of CALB2 mRNA and protein were quantificationally analyzed by qPCR and western blot. Results: CALB2 was mainly located in the cytoplasm of Leydig cells. Sera testosterone level was the lowest in the group A, and the highest in the group B（P＜0.05）. Compared to the group A and the group C, expressions of CALB2 were significantly increased（P＜0.05）. Conclusions: Calretinin is located in the cytoplasm of Leydig cells, and differently expressed in testes of rats at different development stages, which suggested that calretinin regulated testosterone production.

Key words: Androgens, Testis, Leydig cells, Calcium-binding protein, vitamin D-dependent