国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2014, Vol. 33 ›› Issue (6): 415-417.

• 论著 • 上一篇    下一篇

丛生蛋白在人类睾丸精原细胞瘤中表达的研究

徐爱明，刘边疆，王增军   

  1. 210029 南京医科大学第一附属医院泌尿外科
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-11-15 发布日期:2014-11-15
  • 通讯作者: 刘边疆

Expression of Clusterin in Human Testicular Seminoma

XU Ai-ming，LIU Bian-jiang，WANG Zeng-jun   

  1. Department of Urology，the First Affiliated Hospital of Nanjing Medical University，Nanjing 210029，China
  • Received:1900-01-01 Revised:1900-01-01 Published:2014-11-15 Online:2014-11-15
  • Contact: LIU Bian-jiang

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摘要： 目的：探讨丛生蛋白在睾丸精原细胞瘤中的存在和表达。方法：收集13例睾丸精原细胞瘤患者和16例前列腺癌去势术后患者的肿瘤组织及其正常的睾丸组织。免疫组织化学法分析比较2组丛生蛋白存在情况。实时定量聚合酶链反应（RT-PCR）和蛋白质印迹分析（Western blot analysis）分别检测丛生蛋白在转录和翻译水平上的表达差异。结果：RT-PCR结果表明，精原细胞瘤丛生蛋白mRNA的表达低于正常组（仅占正常组织中表达量的13%）。Western blot结果显示在精原细胞瘤中丛生蛋白表达明显低于正常组织。免疫组化结果显示，在正常睾丸组织中丛生蛋白主要表达于间质细胞、支持细胞和成熟的生精细胞，而在精原细胞瘤组织中表达降低。结论：睾丸精原细胞瘤和睾丸正常组织中存在丛生蛋白同种异质二聚体的分泌型。精原细胞瘤睾丸组织中丛生蛋白的表达降低。

关键词: 聚集素, 睾丸肿瘤, 基因表达, 聚合酶链反应, 印迹法, 蛋白质, 免疫组织化学

Abstract: Objective：To explore the expression of clusterin in testicular seminoma tissues. Methods：Tumor tissues were collected from 13 patients with testicular seminoma, and normal testis tissues (as control group) from 16 patients undergoing surgical castration for prostate cancer. Immunohistochemical analysis was performed to compare qualitatively the expression of clusterin in seminoma tissues and control. Real-time polymerase chain reaction（PCR） and western blot analysis were performed to detect quantificationally the expressions of clusterin mRNA and clusterin protein in two groups. Results：The expression of clusterin mRNA in testicular seminoma was significantly lower than that in control group（only 13% of normal tissue）. Analogously, the expression of clusterin protein in testicular seminoma was significantly lower than that in control group. Clusterin expression was mainly located in the Leydig cells, Sertoil cells and maturated spermatogenic cells of normal testes, while there was less expression in seminoma tissues. Conclusions：There were heterodimeric secretory isoforms of clusterin in testicular seminoma and normal testis. The expression of clusterin in testicular seminoma is significantly lowered.

Key words: Clusterin, Testicular neoplasms, Gene expression, Polymerase chain reaction, Blotting, western, Immunohistochemistry