国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2016, Vol. 35 ›› Issue (5): 362-364.

• 论著 • 上一篇    下一篇

玻璃化冷冻的卵母细胞在卵母细胞捐赠中的应用

林玉婷，马翔，蔡令波，曾桥，吴畏，刁飞扬，刘嘉茵，千日成   

  1. 210029 南京医科大学第一附属医院生殖医学科（林玉婷，马翔，蔡令波，曾桥，吴畏，刁飞扬，刘嘉茵，千日成）；南京医科大学生殖医学国家重点实验室（刘嘉茵，千日成）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2016-10-25 发布日期:2016-10-25
  • 通讯作者: 千日成

Application of Human Vitrified Oocytes in Ovum Donation

LIN Yu-ting, MA Xiang, CAI Ling-bo, ZENG Qiao, WU Wei, DIAO Fei-yang, LIU Jia-yin, CHIAN Ri-cheng   

  1. The Clinical Center for Reproductive Medicine,The State Key Laboratory of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029,China
  • Received:1900-01-01 Revised:1900-01-01 Published:2016-10-25 Online:2016-10-25
  • Contact: CHIAN Ri-cheng

PDF (PC)

7843

摘要： 目的：分析玻璃化冷冻的卵母细胞在供卵周期中的临床结局，以期为卵母细胞捐献的临床应用提供参考。方法：回顾性分析2006年2月—2014年10月南京医科大学第一附属医院临床生殖医学中心216个供卵周期的玻璃化冷冻的卵母细胞，比较解冻后的复苏率、行体外受精-胚胎移植（in vitro fertilization-embryo transfer，IVF-ET）的正常受精率、累计临床妊娠率、种植率和累计活产率等临床结局。结果：解冻玻璃化冻存的卵母细胞的复苏率为82.0% （899/1 097），二原核（2PN）受精率76.9%（691/899）。在216个周期中，胚胎种植率为21.4% （84/393），累计临床妊娠率为33.3% （72/216），累计活产率达27.3%（59/216）。每解冻1枚卵母细胞获得活产儿的概率为6.5%（71/1 097）。结论：卵母细胞玻璃化冻融技术在卵母细胞捐赠的临床应用上可以获得较好的临床结局。

关键词: 卵母细胞, 低温保存, 受精, 体外, 胚胎移植, 妊娠结局

Abstract: Objective: To analyse the clinical outcomes of the ovum donation cycles conducted with the vitrified oocytes, so as to provide the predictive information for the clinical application of this technique. Methods: The clinical outcomes of 216 ovum donation cycles, which conducted with the vitrified-thawed oocytes undergoing IVF-ET in our reproductive center from February 2006 to October 2014, were retrospective analyzed. The main outcome parameters were the survival rate of the freezen-thawed oocytes, the normal fertilization（2PN） rate of in vitro fertilization, the cumulative clinical pregnancy rate, the implantation rate and the cumulative live birth rate. Results: The survival rate of the freezen-thawed oocytes was 82.0%（899/1 097）. The normal fertilization （2PN） rate was 76.9%（691/899）. The rates of implantation, cumulative clinical pregnancy and live birth were 21.4%（84/393）, 33.3%（72/216） and 27.3%（59/216）, respectively. The oocyte-to-baby rate was 6.5%（71/1 097）. Conclusions: There is relatively good clinical outcomes of the freezen-thawed oocytes when applied in the ovum donation.

Key words: Oocytes, Cryopreservation, Fertilization in vitro, Embryo transfer, Pregnancy outcome