Schisandrin B Decreases the Effect of PM2.5 on HTR Cell Proliferation

doi:10.12280/gjszjk.20200605

Abstract

Abstract:

Objective: To investigate whether Schisandrin B (Sch B) can attenuate the effect of PM2.5 on the proliferation of human trophoblast HTR-8/Svneo (HTR) cells. Methods: The in vitro cultured HTR cells were treated by PM2.5 of 10-1 000 μg/mL concentration, Sch B-alone and Sch B plus PM2.5. The proliferation of HTR cells was tested by MTS method. Results: PM2.5 of different concentrations from 10 to 1 000 μg/mL inhibited the proliferation of HTR cells (P<0.01), and the inhibition rate of 150 μg/mL PM2.5 was 21.97%. Sch B of the concentration from 0.1 to 5 μmol/L attenuated the PM2.5-induced inhibition of HTR cells proliferation, with a dose-effect relationship among 0.1-2 μmol/L. Sch B of 2 μmol/L presented the highest protection rate (14.07%). Conclusions: Sch B could attenuate the PM2.5-induced inhibition of HTR cell proliferation, suggesting a potential protection of human trophoblast cells in vivo.

Key words: Schisandrin, PM2.5, Trophoblasts, Cell proliferation, Cell culture techniques

LI Xiao, GUO Yan-li, HAN Jun-ting, ZOU Xiao-ping, DONG Qu-long. Schisandrin B Decreases the Effect of PM2.5 on HTR Cell Proliferation[J]. Journal of International Reproductive Health/Family Planning, 2021, 40(3): 177-181.

Figures/Tables 6

References 21

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