Abstract: Infertility is a global reproductive health problem，the number of patients with infertility has
increased year by year. Since the first "test-tube baby" in the world, UK's Louise Brown, was born via in vitro
fertilization（IVF）in 1978，assisted reproductive technology（ART）has been the leading surgical treatment for
couples diagnosed with infertility. ART included artificial insemination（AI）、in vitro fertilization and embryo
transfer（IVF-ET）、intracytoplasmic sperm injection（ICSI）、embryo cryopreservation technology and so on.
Many other ARTs are been rapidly evoluting，such as some ARTs based on human genomics. A series of new
ARTs, such as in vitro oocyte maturation (IVM), preimplantation genetic diagnos（PGD）, single embryo transfer,
gamete and embyo freezing and frost thawing technology, have been developed. These new ARTs provided
possibilities to reduce cost and risk of routine in vitro fertilization and embryo transfer（IVF-ET）. In this paper,
we reviewed the clinical and experimental progress of ARTs

Key words: Reproductive techniques, assisted, Fertilization in vitro, Embryo transfer, Sperm injections, intracytoplasmic, Preimplantation diagnosis, Embryo culture techniques, Cryopreservation