Abstract: Objective：To evaluate the effects of recombinant human luteinizing hormone （rLH）supplementation on the ovarian stimulation and implantation rate in those down -regulated Chinese women ofadvanced reproductive age. Methods :This isa prospective randomized controlled trial. 320 cycles of ART wereanalyzed. All infertile patients with consecutive normogonadotropic function, aged 35-40 years, underwent theirfirst IVF or intracytoplasmic sperm injection （ICSI）treatment cycle. After pituitary suppression with Triptorelinacetate 0.05 mg/d s.c started in the midluteal phase of the previous cycle，the patients were randomized dividedinto five groups：淤control group（n=61）：only received rFSH for ovarian stimulation until day of hCG injection.于T1 （n=63）：rLH 75 IU/d supplementation on day6 of rFSH stimulation until day of hCG injection. 盂T2 （n=65）：rLH 150 IU/d supplementation on day 6 of rFSH stimulation until day of hCG injection. 榆T3 （n=68）：rLH75 IU/d supplementation on day 1 of rFSH stimulation until day of hCG injection. 虞T4 （n=63）：rLH 150 IU/dsupplementation on day1 of rFSH stimulation until day of hCG injection. Ovarian stimulation was initiated withrFSH 225 IU/d, 5 days in all of groups. Results 院No significant differences were observed in terms of numberand maturity of oocytes，fertilized oocytes，implantation rate and pregnancy rate among groups （P ＞0.05）.Conclusions 院The rLH supplementation with daily doses 75 or 150 IU during early follicular phase or secondhalf follicular phase dose not improve ovarian response and implantation rates in those normogonadotropicwomen of advanced reproductive age who were stimulated with rFSH under pituitary suppression with Iriptorelinacetate 0.05 mg/d s.c for IVF，therefore the rLH supplementation is not needed in all down regulated women ofadvanced reproductive age.

Key words: Luteinizing hormone, Fertilization in vitro, Follicle stimulating hormone, human, Controlledovarian stimulation, Infertility, female, Age factors, Pregnancy outcome