Abstract: Objective：To compare the PureSperm technique alone and the PureSperm combined withswim-up method in processing the sperm-binding antibody positive sperm. Methods 院The PureSperm techniquealone and the PureSperm combined with swim -up method were used to process in vitro the sperm -bindingantibody positive sperm from patients with male sterility. Results 院The titer of sperm -binding antibodies wassignificantly declined after treatment by both the PureSperm technique （P＜0.01）and the PureSperm combined with swim -up method （P＜0.01）. The titer of sperm -binding antibodies of the PureSperm alone group wassignificantly lower than that of the PureSperm combination group（P＜0.05）. Conclusions 院The sperm quality can be improved by the PureSperm technique alone or combined with swim -up method in those patients with the positive sperm -binding antibodies. The treatment outcome of IUI for patients with immune infertility can beimproved when used the sperm samples treated with the PureSperm technique.

Key words: Infertility, male, Sperm atozoa, Antibodies, Centrifugation, density gradient, Swim-up method