Abstract: The motile sperm organelle morphology examination（MSOME），a morphological analysis of isolated motile spermatozoa in real-time at more than 6 000 times magnification，can help embryologist to select those sperms with normal morphology for intracytoplasmic sperm injection（ICSI）. MOSME is a very special method to identify sperm head vacuoles. What are sperm vacuoles? Whether may sperm vacuoles influence the outcome of ART? This article reviewed those characteristics of sperm vacuoles as follows：①Vacuoles were commonly found in sperm head; ②Vacuoles were distributed more frequently in the anteromedian region of sperm head; ③There were different types of vacuoles; ④The origin of sperm vacuoles was still unsure; ⑤There were correlation in a certain extent between sperm vacuoles and routine semen parameters. It was found，although unasserted，that sperm vacuoles were positively correlated with abnormal chromatin condensation，DNA fragmentation rate，and aneuploid rate; ⑥Spern vacuoles could lead to the lowed fertilization rate，the lowed rate of high-qualified embryo，and the lowed rate of clinical pregnancy specially.

Key words: Vacuole, sperm, Reproductive techniques, assisted, Sperm injections, intracytoplasmic