Abstract: Gene knockout technology was developed in the second half of the 1980s through the homologous recombinant DNA technology, which played an important role in the research of gene function, developmental biology and diseases. Until now, the homologous recombination DNA technology is still the most popular way to build the knockout models. The knockout mice model has been most widely used. This article mainly reviews some important methods of gene knockout at home and abroad and their application in the study of female reproductive system, including the influence of sex hormone on reproductive organs and hormone-dependent diseases, the mechanisms of cytokines in the regulation of reproductive organs during puberty and gonad development and mature, as well as the defect in the gonadal development of embryo.

Key words: Mice, knockout, Urogenital system, DNA, recombinant, Mutagenesis, insertional, RNA interference