Abstract: Objective：To explore the expression profile of miRNAs related to vascular remodeling of endometrium, and to predict the target genes, as a pioneer research of the pathogenesis of early pregnancy loss (EPL). Methods: The differentially expressed miRNAs in the decidual tissues of EPL were tested using the miRNA microarray, and the expression profile of miRNAs related to vascular remodeling was determined. The target genes of those differentially expressed miRNAs were screened using miRWalk2.0 database. The bioinformatics analysis of these target genes was performed by Gene Ontology analysis (GO-analysis) and Kyoto Encyclopedia of Genes and Genomes Pathway analysis (KEGG Pathway analysis). Results: There were 70 differentially expressed miRNAs in the deciduas tissues of EPL, including 32 up-regulated and 38 down-regulated miRNAs. MiR-125a-5p and miR-29c-3p were two miRNAs with the highest difference times (Ratio＞2.0) in those up-regulated miRNAs, and the predicted target gene was VEGFA. The function of target gene set was mainly located in the positive regulation of RNA metabolism, vascular development, endothelial cell proliferation and hypoxia and angiogenesis. KEGG pathway analysis demonstrated that the gene set was mostly located in mTOR signaling pathway and MAPK signaling pathway. Conclusions: The up-regulated expressions of miR-125a-5p and miR-29c-3p may affect the vascular remodeling in endometrial decidualization by regulating the VEGFA expression, suggesting that miR-125a-5p and miR-29c-3p participate in the pathogenesis of EPL.

Key words: Abortion, spontaneous, Pregnancy trimester, first, MicroRNAs, Computational biology, Vascular endothelial growth factor A, Vascular remodeling