The Safety and Clinical Pregnancy Outcome of Preimplantation Genetic Testing in Frozen-Thawed Embryos

doi:10.12280/gjszjk.20220221

Abstract

Abstract:

Objective: To explore the safety of preimplantation genetic testing (PGT) in frozen-thawed embryos and its effects on the clinical pregnancy outcome. Methods: From August 2016 to December 2021, a total of 95 clinical cases of PGT in frozen-thawed embryos were collected from the Center of Reproductive Medicine, Women’s Hospital of Nanjing Medical University (referred to as the frozen-thawed embryos PGT group, 37 cases had been transplanted up to December 31，2021) and 145 clinical cases of PGT in fresh embryos during the same period (referred to as the fresh-embryos PGT group, 95 cases had been transplanted). The differences in PGT results and the clinical pregnancy outcome of transplanted cases were analyzed between the two groups. Results: ① In 145 cases of the fresh-embryos PGT group, 742 blastocysts were biopsied and 283 euploid embryos were obtained; In 95 cases of the frozen-thawed embryos PGT group, 279 blastocysts were biopsied and 90 euploid embryos were obtained. There was no significant difference in the euploid embryos rates between the two groups (38.14% vs. 32.26%, P=0.082). ② There were no significant differences in the embryo implantation rate(56.84% vs. 54.05%), clinical pregnancy rate (56.84% vs. 54.05%), miscarriage rate (9.26% vs. 15.00%), live birth rate (51.58% vs. 45.95%) and the early birth rate (8.16% vs. 11.76%) between the two groups (all P>0.05). Conclusions: PGT in the frozen-thawed embryos is consistent with PGT in the fresh embryos about diagnostic result and pregnancy outcome. Therefore, PGT in the frozen-thawed embryos can be used as a relatively safe treatment under some special circumstances.

Key words: Embryo, mammalian, Fertilization in vitro, Biopsy, Preimplantation diagnosis, Pregnancy outcome

ZHANG Xiao-lan, WU Xun, CAO Shan-ren, ZHANG Jun-qiang, LING Xiu-feng, LI Xiu-ling. The Safety and Clinical Pregnancy Outcome of Preimplantation Genetic Testing in Frozen-Thawed Embryos[J]. Journal of International Reproductive Health/Family Planning, 2023, 42(2): 102-106.

Figures/Tables 4

References 16

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组别	周期数	年龄（岁）	BMI （kg/m²）	不孕时间（年）	基础FSH （U/L）	促排卵方案			PGT类型
组别	周期数	年龄（岁）	BMI （kg/m²）	不孕时间（年）	基础FSH （U/L）	长方案	拮抗剂方案	PPOS方案	PGT-A	PGT-SR	PGT-M
新鲜PGT组	145	32.22±4.64	21.60±1.99	2.00（1.00，3.46）	7.79±2.13	13.79（20/145）	74.48（108/145）	11.73（17/145）	70.34（102/145）	28.28（41/145）	1.38（2/145）
冻融PGT组	95	32.25±4.48	21.88±2.75	2.25（1.25，4.00）	7.91±2.01	12.63（12/95）	77.90（74/95）	9.47（9/95）	70.53（67/95）	28.42（27/95）	1.05（1/95）
t或Z或 χ²		-0.053	-0.894	-0.923	-0.425		0.415			0.050
P		0.958	0.372	0.356	0.671		0.813			1.000*

组别	周期数	年龄（岁）	BMI （kg/m²）	不孕时间（年）	基础FSH （U/L）	促排卵方案			PGT类型
组别	周期数	年龄（岁）	BMI （kg/m²）	不孕时间（年）	基础FSH （U/L）	长方案	拮抗剂方案	PPOS方案	PGT-A	PGT-SR	PGT-M
新鲜PGT组	145	32.22±4.64	21.60±1.99	2.00（1.00，3.46）	7.79±2.13	13.79（20/145）	74.48（108/145）	11.73（17/145）	70.34（102/145）	28.28（41/145）	1.38（2/145）
冻融PGT组	95	32.25±4.48	21.88±2.75	2.25（1.25，4.00）	7.91±2.01	12.63（12/95）	77.90（74/95）	9.47（9/95）	70.53（67/95）	28.42（27/95）	1.05（1/95）
t或Z或 χ²		-0.053	-0.894	-0.923	-0.425		0.415			0.050
P		0.958	0.372	0.356	0.671		0.813			1.000*

组别	周期数（囊胚数）	整倍体率	非整倍体率	嵌合体率	无结果率	均为异常胚胎的周期率	均为无信号胚胎的周期率	均为异常或无信号胚胎的周期率
新鲜PGT组	145（742）	38.14（283/742）	48.65（361/742）	9.70（72/742）	3.50（26/742）	24.83（36/145）	0（0/145）	2.07（3/145）
冻融PGT组	95（279）	32.26（90/279）	55.20（154/279）	8.24（23/279）	4.30（12/279）	31.58（30/95）	3.16（3/95）	3.16（3/95）
χ²		3.026	3.474	0.512	0.359	1.312	4.637	0.279
P		0.082	0.062	0.474	0.549	0.252	0.119*	0.916*

组别	周期数（囊胚数）	整倍体率	非整倍体率	嵌合体率	无结果率	均为异常胚胎的周期率	均为无信号胚胎的周期率	均为异常或无信号胚胎的周期率
新鲜PGT组	145（742）	38.14（283/742）	48.65（361/742）	9.70（72/742）	3.50（26/742）	24.83（36/145）	0（0/145）	2.07（3/145）
冻融PGT组	95（279）	32.26（90/279）	55.20（154/279）	8.24（23/279）	4.30（12/279）	31.58（30/95）	3.16（3/95）	3.16（3/95）
χ²		3.026	3.474	0.512	0.359	1.312	4.637	0.279
P		0.082	0.062	0.474	0.549	0.252	0.119*	0.916*

组别	囊胚数	整倍体率	非整倍体率	无结果率	嵌合体率	嵌合类型
组别	囊胚数	整倍体率	非整倍体率	无结果率	嵌合体率	单纯型	复杂型
IVF组	126	29.37（37/126）	58.73（74/126）	3.17（4/126）	8.73（11/126）	63.64（7/11）	36.36（4/11）
ICSI组	72	36.11（26/72）	45.83（33/72）	8.33（6/72）	9.72（7/72）	100.00（7/7）	0（0/7）
χ²		0.961	3.068	2.543	0.055	-
P		0.327	0.080	0.209*	0.815	0.119^#