Abstract: Fluorescence in-situ hybridization（FISH） characterized by its simple，rapid，high sensitivity and high specificity has been the preferred method of chromosomal analysis of human sperm. FISH can detect thousands of sperm chromosomes in a batch of analysis. By FISH, it was found that the frequency of aneuploidy in those people contacting with poison, under chemo-treatment, or those people being chromosomal balanced translocation carrier and infertile men, was significantly increased than that in normal men. Especially with the development of intra-cytoplasmic injection （ICSI），application of FISH in detecting sperm chromosomes of infertile men was significantly increased. We hereby reviewed the application of FISH technology in detecting aneuploidy of sperm chromosomes in recent years.

Key words: Fluorescence in-situ hybridization, Spermatozoa, Chromosomes, Aneuploidy, Infertility, male