Abstract: Objective：To investigate diagnosis value of testing P16 and Ki-67 protein expression in
cervical atypical squamous cells undefined significance （ASCUS）. Methods：156 cases who suffered from
ASCUS were collected. Then tested and compared the expression of P16 and Ki-67 in the cervical cell of all
specimen by immunocytochemistry staining and high-risk human papillomavirus （HPV）by nucleic acid
amplification with fluorescence. Results：The positive expression rate of P16 and Ki-67 was positive correlated
with the severity of cervical disease（rs=0.685、rs=0.713，P约0.05）. High-risk HPV-positive rate（59.62%）is
higher than P16, Ki-67 high expression rate（32.5%、33.97%），there are significant difference（字2=30.31 and
25.81, P<0.01）. The sensitivity and specificity of P16，Ki-67 staining in immune cells detecting CIN 域and
above CIN 域lesions were 87.50%，93.75% and 82.26%，81.45%，respectively. The specificity of P16 and
Ki-67 testing in all groups were significantly higher than HPV testing （48.39%，P约0.01). Conclusions：
Detecting P16 and Ki-67 by immunocytotochemical staining had a higher detecting rate of high-evel CIN
compared with a high-risk type HPV testing. It can exclude benign lesions in ASCUS and has a great clinical
signification.

Key words: Cervical intraepithelial neoplasia, Biopsy, Immunohistochemistry, Ki-67 Antigen, Papillomaviridae