Abstract: Objective: To further evaluate the stability of different batches of culture medium by analyzing the IVF outcomes. Methods：Clinical data were collected from August 2011 to September 2012 in our IVF centre. Patients′ characteristics were summarized as younger than 35 years，normal ovarian reserve, using long protocol for ovulation, and more than 5 oocytes per cycles. There were 18 batches of the culture medium according to the cleavage stage （１８ ｇｒｏｕｐｓ）． Six batches （６ ｇｒｏｕｐｓ） were randomly selected. General clinical features and IVF outcomes were compared. Results：There were no significant differences in the average age，the number of previous IVF，basal FSH level，body mass index （BMI）, and endometrial thickness of hCG day, among the six groups（P＞0.05）. There were no significant differences in the average number of oocyte，oocyte maturation rate，fertilization rate，cleavage rate（P＞0.05） among the groups. The high-quality embryo rate was different among the groups（P＜0.05）. However，there were no significant differences in the average number of embryos transferred，pregnancy rate，miscarriage rate among the groups（P＞0.05）. Conclusions：IVF outcomes from different batches of the embryonic culture medium are stable, suggesting the stability of the overall quality control system in our IVF laboratory.

Key words: Reproductive techniques, assisted, Fertilization in vitro, Embryo transfer, Quality control