Abstract: Objective: The differentially expressed proteins in the villous tissues derived from those women of fetal normal chromosome carly embryo damage(EED) and those women asked abortion with non-medical reasons were analyzed with the proteomic method, so as to search some clues of the pathogenesis of EED. Methods: Ten EED patients with fetal normal chromosome (the EED group) and 5 women who asked abortion with non-medical reasons (the control group) were included in this study. The LC-MS/MS, proteomic technology, was use to detect the protein map of villous tissues. The Maxquant software, Uniprot human reference protein database and DAVID Analysis were used to analyse the differential expression profile of proteins. Results: MS experiments identified 3 950 proteins, and qualified 3 879 proteins. A total of 220 proteins were found to be differently expressed including 162 up-regulated proteins and 58 down-regulated proteins in that profile. These proteins were involved in several biological process, such as immune response, reactive oxygen species metabolic process, angiogenesis and so on. The up-regulated C1R, C3, C4A, C4B and C7 were invoved in the activation of complement system, and the up-regulated TPSO was involved in the metabolism of reactive oxygen species. Conclusions: The differential expression profile of proteins was developed. The excessive activation of complement system, and the imbalance of redox reaction, could be related with the pathogenesis of EED.

Key words: Early embryo damage, Proteomics, Complement activation, Reactive oxygen species, Oxidation-reduction