国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2012, Vol. 31 ›› Issue (4): 299-302.

• 综述 • 上一篇    下一篇

单倍体精子细胞卵胞浆内注射研究进展

宋小敏,姜 宏   

  1. 安微医科大学解放军临床学院
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-07-15 发布日期:2012-07-15
  • 通讯作者: 姜 宏

Recent Advances in Study of Microinsemination with Haploid Spermatid

SONG Xiao-min，JIANG Hong   

  1. The 105th Hospital of PLA Affiliated Anhui Medical University，Hefei 230031，China
  • Received:1900-01-01 Revised:1900-01-01 Published:2012-07-15 Online:2012-07-15
  • Contact: JIANG Hong

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摘要： 通过显微注射技术将单倍体精子细胞注射入卵母细胞，如圆形精子细胞注射技术（ROSI）和长形精子细胞注射技术（ELSI），可使非阻塞性无精子症患者获得具有父方遗传物质的后代。单倍体精子细胞可通过精液、睾丸组织活检或生精细胞体外培养获取，但其受精率和妊娠率并不理想。对ROSI/ELSI技术的发展按操作系统、精子细胞注射方式和激活方式、临床应用以及存在的问题进行文献综述。

关键词: 无精子症, 精细胞, 精原细胞, 精子注射, 细胞质内

Abstract: Men with non-obstructive azoospermia might get their offspring with paternal genetic material by micro-injecting haploid spermatid into oocytes （round spermatid injection，ROSI；elongated spermatid injection，ELSI）. The haploid spermatid could be obtained from semen，testis biopsy specimens or in vitro culture of spermatogenic cells. But the fertilization rate and pregancy rate after micro-injecting with haploid spermatids were not satisfactory. In this paper，we discussed the ROSI/ELSI technique and its progress，the spermatid injection and activation，and its clinic application，as well as clinical and technical problems.

Key words: Azoospermia, Spermatids, Spermatogonia, Sperm injections, intracytoplasmic