国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2015, Vol. 34 ›› Issue (2): 106-108.

• 论著 • 上一篇    下一篇

IDO和Foxp3在胚胎反复植入失败患者黄体中期的表达

许思娟，张红红，倪亚莉，谢广妹，高喜红，张霖，刘丽娟   

  1. 730050 兰州，甘肃省妇幼保健院生殖医学中心
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-03-15 发布日期:2015-03-15
  • 通讯作者: 倪亚莉

Expressions of IDO and Foxp3 Genes in Peripheral Blood Mononuclear Cells of Patients with Recurrent Implantation Failure during Midluteal Phase

XU Si-juan，ZHANG Hong-hong，NI Ya-li，XIE Guang-mei，GAO Xi-hong，ZHANG Lin，LIU Li-juan   

  1. Center of Reproductive Medicine，Gansu Provincial Maternity and Child Care Hospital，Lanzhou 730050，China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-03-15 Online:2015-03-15
  • Contact: NI Ya-li

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摘要： 目的：探讨吲哚胺2，3双加氧酶（IDO）和叉头状转录因子3（Foxp3）基因在胚胎反复植入失败中的作用。方法：采用荧光定量实时聚合酶链反应（RT-PCR）技术测定50例胚胎反复植入失败患者（组1）黄体中期Foxp3和IDO基因的表达，并与50例正常生育能力妇女（组2）和50例首次行体外受精-胚胎移植（IVF-ET）并妊娠的妇女（组3）进行比较。结果：3组黄体中期外周血Foxp3 mRNA和IDO mRNA表达比较差异有统计学意义（P＜0.05）；组2和组3 Foxp3 mRNA及IDO mRNA的表达均高于组1（均P=0.000），组2 Foxp3 mRNA及IDO mRNA的表达高于组3（均P＜0.05）。结论：Foxp3 mRNA和IDO mRNA在黄体中期的低表达可能是反复植入失败的免疫性不孕原因。

关键词: 胚胎植入, 双加氧酶类, 叉头转录因子类, RNA, 信使, 黄体期

Abstract: Objective：To investigate the expressions of IDO and Foxp3 genes in the peripheral blood mononuclear cells（PBMCs) of patients with recurrent implantation failure during midluteal phase. Methods：Fifty patients with recurrent implantation failure（group 1）, 50 women with normal fertility（group 2） and 50 women with IVF-ET and pregnancy in their first cycle（group 3） were recruited with consent. Samples of 2 mL peripheral blood were got at their midluteal phase, and mononuclear cells were isolated. Expressions of Foxp3 and IDO mRNA were detected by real-time quantitative-PCR. Results：There was significant difference in expressions of Foxp3 mRNA and IDO mRNA among three groups（P＜0.05）. Expressions of Foxp3 mRNA and IDO mRNA in group 2 and group 3 were significantly higher than those in group 1（P=0.000），while expressions of two genes in group 2 were significantly higher than those in group 3（P＜0.05）. Conclusions：The lowered expressions of Foxp3 mRNA and IDO mRNA in PBMCs of patients during phase may be related with their recurrent implantation failure.

Key words: Embryo implantation, Dioxygenases, Forkhead transcription factors, RNA, messenger, Luteal phase