国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2017, Vol. 36 ›› Issue (6): 477-481.

• 综述 • 上一篇    下一篇

磷脂酶C-zeta与男性不育

陈鑫，刁红录，张昌军，邓锴   

  1. 442000  湖北省十堰市，湖北医药学院附属人民医院生殖医学中心
  • 收稿日期:2017-08-15 修回日期:2017-10-17 出版日期:2017-11-15 发布日期:2017-11-15
  • 通讯作者: 邓锴，E-mail: dkeanig@163.com E-mail:dkeanig@163.com
  • 基金资助:
    国家自然科学基金（81401200）；湖北省教育厅科学技术研究项目（B2015493）

Phospholipase C-zeta and Male Infertility

CHEN Xin, DIAO Hong-lu, ZHANG Chang-jun, DENG Kai   

  1. Department of Reproductive Medicine Center, People′s Hospital Affiliated to Hubei University of Medicine, Shiyan 442000, Hubei Province, China
  • Received:2017-08-15 Revised:2017-10-17 Published:2017-11-15 Online:2017-11-15
  • Contact: DENG Kai, E-mail：dkeanig@163.com E-mail:dkeanig@163.com

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摘要： 卵母细胞激活是胚胎发育的第一步，在哺乳动物中由一系列的细胞内钙离子（Ca2+）振荡触发。精卵融合时一种精子特异性蛋白因子释放入卵母细胞胞质中来启动这些Ca2+振荡，证据表明这种蛋白质是精子特异性磷脂酶C（PLC），称为PLC-zeta（PLCζ）。大量的临床研究显示某些类型的男性不育与PLCζ蛋白在人精子中的异常表达、定位模式、结构改变等显著相关，PLCζ蛋白检测可作为男性不育的诊断和预后指标，显微注射有活性的重组人PLCζ蛋白是人工辅助卵母细胞激活（assisted oocyte activation，AOA）的一种有效方法。本文对PLCζ与卵母细胞激活、PLCζ蛋白的表达水平和定位模式与男性不育以及PLCζ在男性不育诊疗中的临床应用等方面的相关研究进行综述。

关键词: 卵母细胞, Ca2+振荡, 钙, 信号传导, 不育, 男（雄）性, 磷脂酶C-zeta

Abstract: Oocytes activation is the first step of embryonic development, which is triggered by a series of cytoplasmic calcium (Ca2+) oscillations in mammals. Sperm-egg fusion initiates these Ca2+ oscillations by introducing a sperm-specific protein factor into oocytes cytoplasm. This protein factor has been indiated to be sperm-specific phospholipase C (PLC), termed PLC-zeta (PLCζ). Moreover, increasing clinical evidence revealed that some types of male infertility could be related with the aberrant expression of PLCζ and its localization, structure and function in human sperm. PLCζ can be used as a potential diagnostic and prognostic indicator of male infertility. Microinjection of recombinant human PLCζ protein could thus provide an efficient biological solution for inducing artificial activation of oocytes. This review is about the PLCζ and oocytes activation, the PLCζ expression and localization pattern, and male infertility, and advance of PLCζ in the clinical applications of male infertility.

Key words: Oocytes, Ca2+ oscillations, Calcium, Signal transduction, Infertility, male, Phospholipase C zeta