国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2012, Vol. 31 ›› Issue (5): 397-398.

• 论著 • 上一篇    下一篇

梯度离心在体外处理精子结合抗体中的应用

刘莉,岳宗相,赖宇,何维,王世衡,张倡惠   

  1. 611137 成都中医药大学基础医学院微生物免疫教研室（刘莉，岳宗相，赖宁，张倡惠）；成都中医药大学第二附属医院（何维，王世衡）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-09-15 发布日期:2012-09-15
  • 通讯作者: 岳宗相

Application of Gradient Centrifugation In Vitro Treatment of Sperm -binding Antibody

LIU Li，YUE Zong-xiang，LAI Yu，HE Wei，WANG Shi-heng，ZHANG Chang-hui   

  1. Department of Microbiology and Immunol ogy ,Basic Medical College of Chengdu University of TCM ,Chengdu 611137 ,China (LIU Li ,YUE Zong-xiang ,LAI Yu,ZHANG Chang-hui);the Second Affiliated Hospital of Chengdu University of TCM ,Chengdu 610041 ,China (HE Wei ,WANG Shi-heng )
  • Received:1900-01-01 Revised:1900-01-01 Published:2012-09-15 Online:2012-09-15
  • Contact: YUE Zong-xiang

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摘要： 目的：比较单纯PureSperm 方法和PureSperm 联合上游法处理精子结合抗体阳性精子的效果。方法：采用单纯梯度PureSperm 及PureSperm 联合上游法处理精子结合抗体阳性的精子，比较2种处理方法处理后精子结合抗体消退情况。结果：2种方法处理后，精子结合抗体滴度较处理前降低（P＜0.01）；梯度PureSperm 联合上游法处理后，精子结合抗体滴度的下降较单纯PureSperm 方法更显著（P＜0.05）。结论：采用梯度PureSperm 离心或梯度PureSperm 离心联合上游法处理精液，可以提高精子质量，再行宫腔内人工授精可能提高免疫性不育的治疗效果。

关键词: 不育, 男（雄）性, 精子, 抗体, 离心法, 梯密度, 上游法

Abstract: Objective：To compare the PureSperm technique alone and the PureSperm combined withswim-up method in processing the sperm-binding antibody positive sperm. Methods 院The PureSperm techniquealone and the PureSperm combined with swim -up method were used to process in vitro the sperm -bindingantibody positive sperm from patients with male sterility. Results 院The titer of sperm -binding antibodies wassignificantly declined after treatment by both the PureSperm technique （P＜0.01）and the PureSperm combined with swim -up method （P＜0.01）. The titer of sperm -binding antibodies of the PureSperm alone group wassignificantly lower than that of the PureSperm combination group（P＜0.05）. Conclusions 院The sperm quality can be improved by the PureSperm technique alone or combined with swim -up method in those patients with the positive sperm -binding antibodies. The treatment outcome of IUI for patients with immune infertility can beimproved when used the sperm samples treated with the PureSperm technique.

Key words: Infertility, male, Sperm atozoa, Antibodies, Centrifugation, density gradient, Swim-up method