国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2010, Vol. 29 ›› Issue (4): 277-280.

• 综述 • 上一篇    下一篇

胚胎毒性体外试验的研究进展

王雅楠,宋殿荣   

  1. 300193 天津中医药大学（王雅楠）；天津中医药大学 第二附属医院妇科（宋殿荣）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-07-15 发布日期:2010-07-15

Review of in Vitro Embryotoxicity Screening Methods

WANG Ya-nan，SONG Dian-rong   

  1. Tianjin University of Traditional Chinese Medicine Tianjin 300193, China（WANG Ya-nan）；Department of Gynaecology，the Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine，Tianjin 300150，China （SONG Dian-rong）
  • Received:1900-01-01 Revised:1900-01-01 Published:2010-07-15 Online:2010-07-15

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摘要： 胚胎毒性体外试验已广泛应用于胚胎生殖发育毒物筛选和致畸机制研究等方面，现已
有三十余种不同类型的毒性测试方法。欧洲替代方法研究中心推荐的有效性较高的3 个胚胎毒性体外筛
选试验，即体外全胚胎培养试验、胚胎细胞微团培养试验和胚胎干细胞试验具有良好的应用价值，就以上
试验的研究方法、应用价值及研究进展综述。

关键词: 胚胎发育, 毒性试验, 胚胎干细胞, 致畸剂, 胚胎细胞微团培养试验, 体外试验

Abstract: Embryotoxicity tests in vitro, with more than 30 screening methods, are applied on
screening embryotoxicity and developmental toxicity widely. Three effectiveness screening tests recommended
by the European Center for the Validation of Alternative Methods（ECVAM）showed good value of application,
which are whole embryo culture（WEC）、embryonic cell micromass culture（MM）and embryonic stem cell test
（EST）. This article reviews recent progress of embryotoxicity testing in vitro.

Key words: Embryonic development, Toxicity tests, Embryonic stem cells, Teratogens, Limb bud micromass culture, In vitro test