国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2013, Vol. 32 ›› Issue (1): 34-37.

• 综述 • 上一篇    下一篇

人胚胎干细胞向肝脏样细胞诱导分化中限定性内胚层分化的研究进展

孙 博, 孙莹璞   

  1. 450052 郑州大学第一附属医院生殖中心
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2013-01-15 发布日期:2013-01-15
  • 通讯作者: 孙莹璞

Recent Advances of Differentiation of Human Embryonic Stem Cells into Endoderm and Hepato-like Cell Progenitor

SUN Bo，SUN Ying-pu   

  1. Reproductive Medical Center，the First Affiliated Hospital of Zhengzhou University，Zhengzhou 450052，China
  • Received:1900-01-01 Revised:1900-01-01 Published:2013-01-15 Online:2013-01-15
  • Contact: SUN Ying-pu

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摘要： 胚胎干细胞（ESC）是来源于囊胚内细胞团的一种高度未分化细胞，具有无限增殖和发育全能性等特点，可自发分化为多种细胞类型，如神经细胞、肝脏细胞、胰腺细胞和心肌细胞等。在之前的诱导分化方案中，主要通过ESC形成拟胚体后，再进一步进行各种细胞的诱导分化，诱导分化效率较低。目前的研究表明，直接添加诱导因子能够使ESC直接分化成限定性内胚层，并在此基础上进一步诱导分化形成肝脏细胞，从而显著提高向肝脏细胞的诱导分化效率。通过鉴定限定性内胚层的表面标记因子SOX17和FOX2来评定限定性内胚层的诱导分化效率。但是，何种诱导因子的组合诱导分化效率最高并最接近人胚胎正常发育过程并不十分明确。

关键词: 胚胎干细胞, 胚胎, 哺乳动物, 细胞系, 内胚层, 肝细胞

Abstract: Embryonic stem cells （ESC），derived from the inner cell mass，is a highly undifferentiated cells with unlimited proliferation and developmental totipotency. ESC can spontaneously differentiate into many cell types，such as neurocytes，pancreatic cells，cardiocytes，and so on. Previously，ESC were mainly induced to differentiate into a variety of cells by forming the embryoid body （EB），but the efficiency was low. New studies showed that ESC can directly differentiate into definitive endoderm and further into hepatocytes by simply adding inducible factors，by which the efficiency was greatly improved. The efficiency of induced differentiation can be evaluated by the surface markers of definitive endoderm，such as SOX17 and FOX2. However，it is not very clear what combination of inducing factors has the highest efficiency and is closest to the normal development of human embryo.

Key words: Embryonic stem cells, Embryo, mammalian, Cell line, Endoderm, Hepatocytes