国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2015, Vol. 34 ›› Issue (2): 116-118.

• 论著 • 上一篇    下一篇

miRNA-29c和miRNA-30b及其预测靶基因在子宫内膜中的表达

王煜，王丽岩，李光鹏，陈秀娟   

  1. 010050 呼和浩特，内蒙古医科大学附属医院生殖中心
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-03-15 发布日期:2015-03-15
  • 通讯作者: 陈秀娟

Expressions of miRNA-29c，miRNA-30b and Their Target Genes in Endometrium

WANG Yu，WANG Li-yan，LI Guang-peng，CHEN Xiu-juan   

  1. Reproductive Center，Inner Mongolia Medical University Affiliated Hospital，Hohhot 010050，China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-03-15 Online:2015-03-15
  • Contact: CHEN Xiu-juan

PDF (PC)

7612

摘要： 目的：检测子宫内膜和蜕膜组织中miRNA-29c和miRNA-30b及其预测靶基因的表达，探讨miRNA-29c和miRNA-30b在子宫内膜蜕膜化中的意义。方法：选取分泌期子宫内膜组织14例（分泌期组），正常妊娠早期蜕膜组织16例（早孕组）和自然流产蜕膜组织18例（流产组），利用实时荧光定量聚合酶链反应（qPCR）技术检测3组中miRNA-29c和miRNA-30b的表达量，以及候选靶基因血清和糖皮质激素诱导蛋白激酶1（serum and glucocorticoid-inducible kinase 1，SGK1）和瘦素受体（leptin receptor，LEPR）的mRNA表达量，分析miRNA-29c和miRNA-30b表达与SGK1和LEPR的关系。结果：流产组中miRNA-29c的表达量高于分泌期组，差异有统计学意义（P＜0.05）。3组miRNA-30b的表达差异无统计学意义（P＞0.05）；SGK1和LEPR在早孕组表达最高，流产组中表达最低，差异有统计学意义（P＜0.05）。结论：miRNA-29c的表达变化可能通过靶向SGK1参与子宫内膜蜕膜化。SGK1和LEPR在蜕膜中的差异表达对维持早期妊娠有一定的作用。

关键词: miRNAs, 血清和糖皮质激素诱导蛋白激酶1, 受体, 瘦素, 子宫内膜

Abstract: Objective：To detect the expressions of miRNA-29c，miRNA-30b and those predictive target genes in human endometrium and decidua tissues，and to explore their effects on decidualization of endometria. Methods：Tissue samples including 14 secretory endometria，16 early pregnancy deciduas and 18 spontaneous miscarriage deciduas were collected. Expressions of miRNA-29c and miRNA-30b were detected by quantitative real-time polymerase chain reaction（ｑＰＣＲ）. Expressions of two predicted target genes of miRNA-29c and ｍｉＲＮＡ－３０ｂ， ｓｅｒｕｍ ａｎｄ ｇｌｕｃｏｃｏｒｔｉｃｏｉｄ－ｉｎｄｕｃｉｂｌｅ ｋｉｎａｓｅ １（ＳＧＫ１） ａｎｄ lｅｐｔｉｎ rｅｃｅｐｔｏｒ （ＬＥＰＲ）， ｗｅｒｅ ａｌｓｏ detected by qPCR. Results：The expression of miRNA-29c in the spontaneous miscarriage decidua tissues was higher than that in the secretory endometrial tissues（P＜0.05）. There were not significant differences in expressions of miRNA-30b among three groups（P＞0.05）. Moreover，expressions of SGK1 and LEPR mRNAs were at highest level in the early pregnancy deciduas，while they were at lowest level in the spontaneous miscarriage deciduas（P＜0.05）. Conclusions：Expression of miRNA-29c in endometrium may be associated with decidualization by targeting SGK1. Expressions of SGK1 and LEPR in deciduas tissue may be associated with maintaining early pregnancy.

Key words: MicroRNAs, Serum and glucocorticoid-inducible kinase 1, Receptors, leptin, Endometrium