国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2014, Vol. 33 ›› Issue (1): 2-4.

• 论著 • 上一篇    下一篇

裸核注入法在小鼠体细胞核移植中的应用

史三宝，纪冬梅，陈蓓丽，陈大蔚，郝燕，周平，章志国，曹云霞   

  1. 230022 合肥，安徽医科大学第一附属医院生殖中心
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-01-15 发布日期:2014-01-15
  • 通讯作者: 曹云霞

Application of the Bare Nuclear Injection in Mouse Somatic Cell Nuclear Transfer

SHI San-bao，JI Dong-mei，CHEN Bei-li，CHEN Da-wei，HAO Yan，ZHOU Ping，ZHANG Zhi-guo，CAO Yun-xia   

  1. Reproductive Center，the First Affiliated Hospital of Anhui Medical University，Hefei 230022，China
  • Received:1900-01-01 Revised:1900-01-01 Published:2014-01-15 Online:2014-01-15
  • Contact: CAO Yun-xia

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摘要： 目的：探讨供核细胞的处理方式对小鼠体细胞核移植胚胎体内外发育潜能的影响。方法：对供核细胞采用供体胞浆内完整注入和裸核注入两种不同的注核方式，观察重构胚的激活、卵裂及囊胚发育情况；并设立体外受精组通过胚胎受体内移植观察2种注核方式所获重构胚的发育结局。结果：完整细胞组的卵裂率和囊胚率均低于裸核组，差异有统计学意义（χ2卵裂=5.785，P=0.016；χ2囊胚=6.092，P=0.014）；3组所获仔鼠质量比较差异有统计学意义（F=14.331，P=0.00），体外受精组最轻，完整细胞组最重，而完整细胞组所获得胎盘的质量高于裸核组及体外受精组，差异有统计学意义（与体外受精组相比，t=-8.786，P=0.00；与裸核组相比，t=-6.573，P=0.00）。结论：裸核注入法可提高小鼠体细胞核移植胚胎的体内外发育潜能。

关键词: 核移植技术, 遗传学技术, 分裂期, 卵, 囊胚, 胚胎发育

Abstract: Objective: To investigate the developmental potential of mouse somatic nuclear transfer embryos （SCNT） with different treatment of donor cell. Methods：Two methods, the intact cell and the naked nuclei injection，were used to produce cloned embryos. The reconstructed embryos and IVF control embryos were transferred into receptors′ uterine cavity. The rates of activation，cleavage，blastocyst of reconstructed embryos and in vivo developmental outcomes were compared. Results：The rate of cleavage and blastocyst in the intact cell group was significantly lower than that in the bare nucleus group （χ2cleavage=5.785，P=0.016；χ2blastocyst=6.092，P=0.014）. There were significantly difference in the weight of fetus in three groups F=14.331，P=0.00），the intact group had the heaviest wet weight of fetus. The weights of placental in the bare nuclear group and the IVF group were significantly lower than that in the intact cell group （compared with the normal group，t=-8.786，P=0.00；compared with bare nuclear group，t=-6.573，P=0.00）. Conclusions：The developmental potential of mouse somatic cell nuclear transfer embryos could be improved by the bare nucleus injection.

Key words: Nuclear transfer techniques, Genetic techniques, Cleavage stage, ovum, Blastula, Embryonic development