国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2013, Vol. 32 ›› Issue (3): 154-156.

• 论著 • 上一篇    下一篇

二甲双胍对卵巢颗粒细胞IRS-1及ERK-2基因表达的影响

任文超 ,刘建新, 祁秀娟, 丁 钰, 王 琳   

  1. 266061 青岛大学医学院附属医院生殖中心
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2013-05-15 发布日期:2013-05-15
  • 通讯作者: 刘建新

Effect of Metformine on the Expression of Insulin Receptor Substrates-1 and Extracellular Signal-Regulated Kinases-2 in Granulosa Cells

REN Wen-chao，LIU Jian-xin，QI Xiu-juan，DING Yu,WANG Lin   

  1. Department of Reproductive Medicine， the Affiliated Hospital of Medical College Qingdao University，Qingdao 266061，China
  • Received:1900-01-01 Revised:1900-01-01 Published:2013-05-15 Online:2013-05-15
  • Contact: LIU Jian-xin

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摘要： 目的：探讨二甲双胍对多囊卵巢综合征（PCOS）卵巢颗粒细胞胰岛素受体底物-1（IRS-1）及细胞外信号调节激酶-2（ERK-2）的基因表达的影响。方法：收集行体外受精-胚胎移植（IVF-ET）治疗的PCOS患者卵泡颗粒细胞进行体外培养，用二甲双胍处理48 h，采用实时荧光定量聚合酶链反应（PCR）测定颗粒细胞IRS-1及ERK-2基因的相对表达量，另收集行IVF-ET非PCOS患者颗粒细胞作为对照组。结果：PCOS患者颗粒细胞IRS-1基因、ERK-2基因相对表达量与对照组相比明显升高（P＜0.05），二甲双胍处理后PCOS患者颗粒细胞IRS-1基因相对表达量升高、而ERK-2基因表达量下降（P＜0.05）。结论：PCOS患者颗粒细胞IRS-1与ERK-2基因受二甲双胍作用时表达不同步，二甲双胍可能通过降低PCOS患者颗粒细胞丝裂原活化蛋白激酶（MAPK）信号传导通路活性，减轻MAPK信号通路对磷脂酰肌醇3 激酶（PI3K）通路的负反馈作用，改善PCOS患者卵巢胰岛素抵抗状态。

关键词: 多囊卵巢综合征, 受精, 体外, 胚胎移植, 受体, 胰岛素, 细胞外信号调节MAP激酶类

Abstract: Objective： To study the effect of metformoin on the expression of insulin receptor substrate-1 （IRS-1） and extracellular signal-regulated kinase-2 （ERK-2） in ovarian granulosa cells of the patients with polycystic ovarian syndrome （PCOS）． Methods：Granulosa cells were collected from PCOS patients during IVF-ET. Those granulosa cells from non-PCOS patients during IVF-ET were used as control. After in vitro treated by metformin for 48 h, the cultured granulose cells were examined for IRS-1 and ERK-2 mRNA expression by real-time quantitative PCR. Results：The expression levels of IRS-1 and ERK-2 mRNAs in the granulosa cells of PCOS patients were higher than those in control group （Ｐ＜０．０５）. The level of IRS-1 mRNA was increased by metformoin treatment, while the level of ERK-2 mRNA was decreased（P＜0.05）. Conclusions：Metformoin may alleviate the pathological state of insulin resistance in ovarian of PCOS patients by decreasing the activity of MAPK insulin signaling pathway, which has a negative effect on PI3K pathway.

Key words: Polycystic ovary syndrome, Fertilization in vitro, Embryo transfer, Receptor, insulin, Extracellular signal-regulated MAP kinases