Journal of International Reproductive Health/Family Planning

Journal of International Reproductive Health/Family Planning ›› 2012, Vol. 31 ›› Issue (4): 265-267.

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Analysis on the Aneuploidy Incidence of Chromosome X/Y and 18 in Later Cleavaged Embryos

SHI Jie，YANG Qing-xiu，LUO Hai-ning，ZHANG Yun-shan   

  1. Tianjin Central Hospital of Obstetrics and Gynecology, Tianjin 300100, China（SHI Jie，LUO Hai-ning，ZHANG Yun-shan）；Department of Obstetrics and Gynecology, the Affiliated Hospital of Chinese People’s Armed Forces Logistic College, Tianjin 300162，China（YANG Qing-xiu）
  • Received:1900-01-01 Revised:1900-01-01 Published:2012-07-15 Online:2012-07-15
  • Contact: ZHANG Yun-shan

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Abstract: Objective： To analyze the aneuploidy incidence of Chromosome X，Y and 18 in later cleaved embryos. Methods：The aneuploidy of Chromosome X，Y and 18 in later cleaved embryos from MⅡoocytes and two pronuclei （2PN） zygotes was detected by the method of fluorescence in situ hybridization （FISH）. Results：There were 45 later cleaved embryos from MⅡoocytes and 40 poor scored embryos from 2PN zygotes among 85 obtained embryos. Fluorescent signals were successfully catched in 512 nuclei. The abnormality rates of X，Y and 18 chromosomes were 34.5% and 38% in later cleaved embryos，and 32% and 39% in poor scored embryos from 2PN zygotes. The differences of aneuploidy incidences of chromosome X，Y and 18 were statistically insignificant between two groups. Conclusions：There are high incidences of Chromosome X，Y and 18 in later cleaved embryos and poor scored embryos from 2PN zygotes， showing that those embryos are not suitable for embryo transfer.

Key words: In situ hybridization, fluorescence, Sex chromosomes, Chromosomes, human, pair 18, Aneuploidy, Two pronuclei zygotes