Abstract: Objective： To evaluate the clinical application of three steps screening for cervical cancer, being thinprep cytology test （TCT），high-risk human papilloma virus （HR-HPV） DNA，and colposcopy biopsy for pathological examination. Methods：The screening results of 8 199 women who received cervical cancer screening at Chanchcheng Central Hospital of Foshan，from January 2009 to December 2011，with the three steps screening program，were analyzed retrospectively. Results：The positive rate of TCT was 6.6%，and the positive rate of ≥HSIL was 1.56%，in 8 199 participants. The infection rate of HR-HPV was 55.96% in 1 258 participants. 783 participants received colposcopy，and the positive rate was 56.32%. 441 patients underwent cervical biopsy，the biopsy rate was 5.38%. In pathological examination，the positive rate of precancer was 51.93%，the detection rate of high-grade cervical intraepithelial neoplasia was 25.4%. According to pathological grades，the detection rates were：CINⅠ54.70%，CINⅡ86.49%， CINⅢ96.43%，cervical cancer 97.87%. The detection rates of CINⅠand cervical cancer were higher than that of CINⅢ，while the double positive rate of TCT and HR-HPV DNA of cervical cancer was statistically higher than those of CINⅠ，CINⅡ and CINⅢ （P＜0.01）. Conclusions： Three steps screening，TCT combining with HR-HPV DNA detection and colposcopy in cervical biopsy for pathology，can effectively detect cervical lesions and improve screening rate of cervical cancer.

Key words: Uterine cervical neoplasms, Multiphasic screening, Cytological techniques, Colposcopy, Human papillomavirus, Biopsy