Abstract: According to the WHO guideline, tests for antisperm antibody （ASAB）on spermatozoa were recommended as: the mixed antiglobulin reaction（MAR），the immunobead test（IBT）and sperm cervical mucus contact test（SCMC）. These tests can detect ASAB on mobile spermatozoa or in biological fluids such as serum，seminal plasma and cervical mucus and so on. Vasectomized men were the good model for the ASAB research.We used IBT and SCMC to detect ASAB on the surface of spermatozoa from the proximal vas deferens ofvasectomized men and the semen after vasovasostomy. The relationship between ASAB and infectious diseases ofthe male reproductive tract，varicocele and cryptorchidism were reviewed, so as to show the importance ofscreening semen ASAB in diagnosis of male infertility.

Key words: Autoantibodies, Spermatozoa, Sterilization, tubal, Male, Infection