Abstract: Objective： To construct the Hsa miR-7 lentiviral vector, and to detect its effectiveness in human ovarian cancer cell line HO8910-PM. It is the bases of functional study of Hsa miR-7 in ovarian cancer. Methods：Hsa miR-7 was amplified from the genomic DNA and inserted into pLVX-IRES-ZsGreen1 vector after double digestion to generate pLVX-miR7-IRES-ZsGreen1 vector. The vector was then confirmed by PCR and DNA sequencing. HO8910-PM was infected by the concentrated lentivirus 293T produced. Pre-miR-7，miR-7 and gene EGFR were assessed by qPCR. Results：The recombinant lentiviral vector was successfully established and confirmed by PCR and DNA sequencing. Pre-miR-7 （t=17.909，P=0.004） was integrated into the genome of HO8910-PM and miR-7 （t=35.320，P=0.024） expression was enhanced effectively by qPCR. Moreover，miR-7 target gene EGFR was also reduced both in mRNA （t=8.83，P=0.005） and protein （t=22.14，P=0.002） levels after miR-7 overexpression in HO8910-PM. Conclusions：The Hsa miR-7 lentiviral expression vector was successfully constructed，and the Hsa miR-7 overexpressed in HO8910-PM cell line.

Key words: MicroRNAs, Lentivirus, Ovarian neoplasms, Receptors, vascular endothelial growth factor