Abstract: Objective: To evaluate the levels of estradiol，progesterone and luteinizing hormone（LH） in human cervical mucus around ovulation. Methods：Human cervical mucus and serum samples were collected from twenty adult heathy women during the peri-ovulation. Levels of three hormones were tested using the chemiluminescent immunoassay. Ovulation was confirmed by B ultrasound. Results：The secreting models of the estradiol，progesterone and LH existed in human cervical mucus were similar with those in peripheral blood. There were the estradiol peak and the LH peak on the 1 day before ovulation. The level of progesterone was elevated from this day. However, the levels of three hormones in cervical mucus were significantly lower than those in blood. Conclusions：It could be beneficial to predict ovulation to test the sexual hormones existed in cervical mucus during periovulation.

Key words: Natural family planning methods, Cervix mucus, Estrogens, Progestins, Luteinizing hormone, Ovulation