Abstract: Objective: To observe the the ovarian oxidative stress in mice undergoing different degrees of stimulation, as a theoretical reference for adjuvant psychotherapy of female infertility. Methods：Eighty female mice were randomly divided into 4 groups. The unpredictable stimulation at different degrees was given for 4 weeks continuously. The body weight and behavioral changes were observed to confirm whether the model of psychological stress was successfully prepared. The levels of serum SOD and ROS, the ovarian SOD activity and MDA, and the levels of ovarian SOD2, CAT and GSH-Px mRNAs were detected. Results：The body weight was decreasedin the psychological stress groups when compared with the control group, the immobility time and the central area retention time were prolonged, and the number of times entering the central area and the central area activity time were decreased. The level of serum ROS was increased, while the level of serum SOD was decreased. In the ovarian tissues, the activity of SOD was decreased and the level of MDA increased, while the activities of SOD2, CAT and GSH-Pxwere decreased. Conclusions：Psychological stress can reduce the desire for independent activities and exploration. The ROS accumulation can lead to oxidative stress and affect female reproductive function.

Key words: Stress, psychological, Oxidative stress, Reactive oxygen species, Superoxide dismutase, Mice