Abstract: Objective：To evaluate the cryopreservation effects of the programmed freezing and the vitrification freezing on human ovarian tissue. Methods: Human ovarian tissue specimens of 19 endometrial carcinoma cases were collected. The tissue specimen was divided into two parts: one was cryopreserved with the programmed freezing (the group A), and another with the vitrification freezing （ｔhe group B）． 38 SCID nude mice（transplanted host）were equally divided into two groups，and the thawed human ovarian tissues from the two groups were transplanted to jugular subcutaeous. The day of transplanted host received ovariectomy was defined as D0, while the day of human ovarian tissue transplanted defined as D21. The vaginal cytology examination of transplanted host was performed daily for observation of estrous cycle until D63. The levels of serum AMH on D0, D21, D42 and D63 were measured using ELISA. Results: The estrous cycle of the ovariectomy mice disappeared. A total of 14 transplanted hosts in two groups recovered the estrous cycle up to D63，and there was no significant difference between the two groupｓ （８ in the group A and 6 in the group B, P＞0.0５）． However, the recovering time of the estrous cycle in the group A was significantly shorter than that in the group B [(16.38±1.60) d vs. (18.33±1.21)d, P＜0.05]． There were no significant differences in the AMH levels on D0, D21, D42 and D63 between the two groupｓ （Ｐ＞0.05）． The AMH level on D21 was significantly lower than that on D0 in the group A or B (all P<0.05). The AMH level in those transplanted hosts who had recovered estrous cycle on D42 and D63 was recovered but did not return to the level on D0, while the AMH level in the transplanted hosts who had not recovered their estrous cycle on D42 and D63 did not rebound and was still significantly lower than that on D0. Conclusions: Both the programmed freezing and the vitrification freezing can effectively preserve ovarian tissue.

Key words: Ovary, Cryopreservation, Anti-mullerian hormone, Programmed freezing, Vitrification freezing, Fertility