The Influence of Perinatal Exposure of Low Dose of Bisphenol A on Spermatogenesis of Male Offspring Rats

doi:10.12280/gjszjk.20210097

Abstract

Abstract:

Objective: To study the influence of perinatal exposure of bisphenol A (BPA) at a low dose on the spermatogenesis of male offspring rats. Methods: Pregnant rats were injected subcutaneously with BPA 2 μg/(kg·d) from gestation day 10 through lactation day 7, and the same volume of olive oil was used as the blank control. Their male offspring were served as the BPA group and the control group. Histological structure of the testis and the related regulation genes such as cyclin A1、cyclin B、c-jun and c-fos, as well as the FSH and LH, were tested, and the expressions POA-GnRH or ARC-kiss1 mRNAs were also examined in their male offspring during the postnatal day 18, 21, 24. Results: In comparison with the control group, the seminiferous tubular diameter, the number of round spermatids and the ratio of seminiferous tubule with round spermatids were significantly increased in PND24 BPA rats (P<0.05). The expression levels of cyclin A1, c-jun and c-fos mRNAs in the testes were significantly elevated in PND21 BPA rats (P<0.01). Similarly, the levels of plasma FSH and LH were increased in the PND21/24 BPA rats compared with the control rats (P<0.05). But the levels of plasma T and E₂ were no significant in the PND18/21/24 BPA rats and the control rats (P>0.05). In comparison with control group, the expression levels of POA-GnRH mRNA and ARC-kiss1 mRNA were significantly increased in PND21 BPA rats (GnRH: P<0.05; kiss1: P<0.01). Conclusions: In addition to the direct effect of BPA on the testes of male offspring, the perinatal exposure to a low dose of BPA promotes spermatogenesis via advancing the pubertal activation of ARC-kisspeptin, and via further enhancing the expressions of meiotic regulation genes in the testes of male offspring.

Key words: Spermatogenesis, Peripartum period, Meiosis, Hypothalamus, Animal experimentation, Rats, Bisphenol A

BAI Yin-yang, XIONG Fang, ZHANG Yun, CHEN Jie, XU Li-shuang, WANG Min. The Influence of Perinatal Exposure of Low Dose of Bisphenol A on Spermatogenesis of Male Offspring Rats[J]. Journal of International Reproductive Health/Family Planning, 2021, 40(5): 353-358.

Figures/Tables 6

References 20

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基因名称	上游引物（5′—3′）	下游引物（5′—3′）	大小（bp）
kiss1	CTTCTCCTCTGTGTGGCCTCTTTTG	GGAGTTCCAGTTGTAGGCTGACATG	312
GnRH	CTGTTGTTCTGTTGACTGTGTGTTTGG	CATCTTCTTCTGCCCAGCTTCCTC	248
cyclin A1	GTTCCAACCACCAACCAGTTCCTC	CTCACTCAGGCAAGGCACAATCTC	255
cyclin B	CACCAAGCACCAGATCAGACAGATG	ACGGTTCACCATGACTATGTTCTTAGC	346
c-jun	CAATGGGCACATCACCACTACACC	TTGAAGTTGCTGAGGTTGGCGTAG	279
c-fos	AAGCGGAGACAGATCAACTTGAAGAC	CATTGAGAAGAGGCAGGGTGAAGG	240
GAPDH	GGTGATGCTGGTGCTGAGTATGTC	CAGTCTTCTGAGTGGCAGTGATGG	301

基因名称	上游引物（5′—3′）	下游引物（5′—3′）	大小（bp）
kiss1	CTTCTCCTCTGTGTGGCCTCTTTTG	GGAGTTCCAGTTGTAGGCTGACATG	312
GnRH	CTGTTGTTCTGTTGACTGTGTGTTTGG	CATCTTCTTCTGCCCAGCTTCCTC	248
cyclin A1	GTTCCAACCACCAACCAGTTCCTC	CTCACTCAGGCAAGGCACAATCTC	255
cyclin B	CACCAAGCACCAGATCAGACAGATG	ACGGTTCACCATGACTATGTTCTTAGC	346
c-jun	CAATGGGCACATCACCACTACACC	TTGAAGTTGCTGAGGTTGGCGTAG	279
c-fos	AAGCGGAGACAGATCAACTTGAAGAC	CATTGAGAAGAGGCAGGGTGAAGG	240
GAPDH	GGTGATGCTGGTGCTGAGTATGTC	CAGTCTTCTGAGTGGCAGTGATGG	301

检测时间	组别	n	生精小管直径（μm）	支持细胞体积（mm³）	精原细胞体积（mm³）	精母细胞体积（mm³）	圆形精子细胞管腔率（%）	圆形精子细胞数量
PND18	对照组	10	91.12±8.55	4.91±0.75	0.41±0.07	0.34±0.06	0	0
	BPA组	10	93.43±7.71	5.39±0.55	0.38±0.06	0.32±0.07	0	0
	t		-0.63	-1.67	1.04	0.75	-	-
	P		0.54	0.11	0.31	0.46	-	-
PND21	对照组	10	108.73±5.59	5.41±0.66	0.44±0.06	0.74±0.08	0	0
	BPA组	10	110.84±6.07	5.98±0.71	0.39±0.08	0.81±0.08	0	0
	t		-0.80	-1.87	1.49	-1.80	-	-
	P		0.43	0.08	0.15	0.09	-	-
PND24	对照组	10	131.03±8.21	7.71±0.73	0.57±0.09	1.31±0.10	10.31±2.67	100.40±11.97
	BPA组	10	140.82±9.34	8.13±0.84	0.50±0.08	1.39±0.11	20.52±3.78	410.50±41.78
	t		-2.49	-1.14	1.69	-1.50	-6.97	-22.55
	P		0.02	0.27	0.11	0.15	0.00	0.00

检测时间	组别	n	生精小管直径（μm）	支持细胞体积（mm³）	精原细胞体积（mm³）	精母细胞体积（mm³）	圆形精子细胞管腔率（%）	圆形精子细胞数量
PND18	对照组	10	91.12±8.55	4.91±0.75	0.41±0.07	0.34±0.06	0	0
	BPA组	10	93.43±7.71	5.39±0.55	0.38±0.06	0.32±0.07	0	0
	t		-0.63	-1.67	1.04	0.75	-	-
	P		0.54	0.11	0.31	0.46	-	-
PND21	对照组	10	108.73±5.59	5.41±0.66	0.44±0.06	0.74±0.08	0	0
	BPA组	10	110.84±6.07	5.98±0.71	0.39±0.08	0.81±0.08	0	0
	t		-0.80	-1.87	1.49	-1.80	-	-
	P		0.43	0.08	0.15	0.09	-	-
PND24	对照组	10	131.03±8.21	7.71±0.73	0.57±0.09	1.31±0.10	10.31±2.67	100.40±11.97
	BPA组	10	140.82±9.34	8.13±0.84	0.50±0.08	1.39±0.11	20.52±3.78	410.50±41.78
	t		-2.49	-1.14	1.69	-1.50	-6.97	-22.55
	P		0.02	0.27	0.11	0.15	0.00	0.00