国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2014, Vol. 33 ›› Issue (5): 334-338.

• 论著 • 上一篇    下一篇

机械刮宫法建立比格犬宫腔粘连模型

曹兴凤，王蔼明，尹善德，赵军，赵勇，王明凯   

  1. 100048 中国人民解放军海军总医院[曹兴凤（现工作于江苏省无锡市妇幼保健院），王蔼明，尹善德，赵军，赵勇，王明凯]
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-09-15 发布日期:2014-09-15
  • 通讯作者: 王蔼明

Animal Model of Intrauterine Adhesions Developed by Mechanical Curettage

CAO Xing-feng，WANG Ai-ming，YIN Shan-de，ZHAO Jun，ZHAO Yong，WANG Ming-kai   

  1. Navy General Hospital，Beijing 100048，China［CAO Xing-feng（now working at Wuxi Maternal and Child Health Hospital of Jiangsu Province），WANG Ai-ming，YIN Shan-de，ZHAO Jun，ZHAO Yong，WANG Ming-kai］
  • Received:1900-01-01 Revised:1900-01-01 Published:2014-09-15 Online:2014-09-15
  • Contact: WANG Ai-ming

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摘要： 目的：通过机械刮宫法建立稳定的比格犬宫腔粘连的动物模型，以利于进一步研究探索。方法：6只性成熟的健康雌性比格犬的子宫按处理方法不同分为4组。所有经过搔刮处理后2个月的犬右侧子宫角组织作为处理组，搔刮前该部位正常内膜组织、同侧未处理的子宫组织及左侧未处理的相应部位的子宫组织为对照组，分别记为搔刮前组、同侧未处理组、对侧组。通过机械刮宫法损伤右侧子宫角内膜，分别于造模前后收集子宫内膜标本，行苏木精-伊红染色法、Masson染色法及免疫组织化学染色法并进行分析。结果：处理组外观异常的发生率高于对侧组（P＜0.05）；处理组的内膜厚度薄于搔刮前组和对侧组（P＜0.05）；处理组的内膜腺体数目明显比搔刮前组、同侧未处理组和对侧组少（P＜0.05）；处理组的子宫内膜纤维化面积比例显著高于搔刮前组、同侧未处理组和对侧组（P＜0.001）；胰岛素生长因子Ⅰ（IGF-Ⅰ）、转化生长因子β1（TGF-β1）在处理组内膜上的表达水平高于搔刮前组、同侧未处理组和对侧组（P＜0.05），碱性成纤维细胞生长因子（bFGF）在处理组内膜上的表达水平高于搔刮前组（P＜0.05）。结论：利用机械刮宫法可以建立稳定的比格犬宫腔粘连模型，而子宫内膜组织中的IGF-Ⅰ、TGF-β1和bFGF的异常表达可能参与宫腔粘连的形成。

关键词: 黏连, 刮宫术, 模型, 动物, 犬科

Abstract: Objective：To develop a stable beagle model of intrauterine adhesions（IUA） by mechanical curettage. Methods：The uterine tissues from six sexual maturity and healthy female beagle dogs were divided into four groups：Group 1，distal endometrial samples of the right uterus from those beagle dogs treated by mechanical curettage method, after two months；Group 2，distal samples of the left uterus without treatment；Group 3，proximal samples of the right uterus without treatment；Group 4，proximal samples of the left uterus without treatment. Samples were analyzed by HE staining, Masson staining and immunohistochemistry. Results：The incidence of abnormal endometrial appearance in the Group 1 was obviously higher than that of the Group 2（P＜0.05）. The endometrial thickness of the Group 1 was obviously thinner than those of Group 3 and Group 2（P＜0.05）. The number of endometrial gland of the Group 1 was significantly less than those of other groups（P＜0.05），while the area of endometrial fibrosis was also significantly bigger（P＜0.001）. The expression levels of IGF-Ⅰ and TGF-β1 in the Group 1 were significantly higher than those in other three groups（P＜0.05），while the expression level of bFGF was significantly higher than that in the Group 2（P＜0.05）. Conclusions：A stable beagle model of IUA was successfully developed by the mechanical curettage. The increased expressions of IGF-Ⅰ，bFGF and TGF-β1 may be involved in IUA in this model.

Key words: Adhesions, Dilatation and curettage, Models, animal, Canidae