国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2016, Vol. 35 ›› Issue (1): 51-55.

• 综述 • 上一篇    下一篇

基因敲除在雌性小鼠生殖系统研究中的应用

秦智娟，杨晓清，张玉泉   

  1. 226001 南通大学附属医院妇产科
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2016-01-15 发布日期:2016-01-15
  • 通讯作者: 张玉泉

Application of Knockout Female Mice in Reproductive System Research

QIN Zhi-juan，YANG Xiao-qing，ZHANG Yu-quan   

  1. Department of Obstetrics and Gynecology，Affiliated Hospital of Nantong University，Nantong 226001，Jiangsu Province，China
  • Received:1900-01-01 Revised:1900-01-01 Published:2016-01-15 Online:2016-01-15
  • Contact: ZHANG Yu-quan

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摘要： 基因敲除技术是20世纪80年代后半期应用DNA同源重组技术发展起来的，在对基因功能、发育生物学和疾病的研究中发挥了重要作用。直到现在，运用同源重组实现基因敲除依然是构建基因敲除动物模型中最普遍最经典的方法。基因敲除动物模型中应用最广泛的是基因敲除小鼠模型。综述目前国内外实现基因敲除的几种重要方法以及利用同源重组实现的基因敲除技术在雌性小鼠生殖系统研究中的应用。这些应用主要包括研究性激素对生殖器官及激素依赖性疾病的影响、细胞分泌因子在发育期调控生殖器官组织的机制及胚胎形成后的性腺发育与成熟缺陷等。

关键词: 小鼠, 基因敲除, 泌尿生殖系统, DNA, 重组, 诱变, 插入, RNA干扰

Abstract: Gene knockout technology was developed in the second half of the 1980s through the homologous recombinant DNA technology, which played an important role in the research of gene function, developmental biology and diseases. Until now, the homologous recombination DNA technology is still the most popular way to build the knockout models. The knockout mice model has been most widely used. This article mainly reviews some important methods of gene knockout at home and abroad and their application in the study of female reproductive system, including the influence of sex hormone on reproductive organs and hormone-dependent diseases, the mechanisms of cytokines in the regulation of reproductive organs during puberty and gonad development and mature, as well as the defect in the gonadal development of embryo.

Key words: Mice, knockout, Urogenital system, DNA, recombinant, Mutagenesis, insertional, RNA interference