Abstract: Preimplantation genetic diagnosis (PGD) is an important technology of the assisted reproductive technology (ART). The diagnosis of chromosomal abnormality is an important part of PGD. For thein vitro fertilization embryos of Robertsonian translocation carriers，fluorescence in situ hybridization (FISH) asa PGD technology can be used to solve the fertility problem, to reduce genetic risk and achieve the eugenicpurposes. This review summarized current knowledge of FISH and its application in PGD of the Robertsoniantranslocation carriers.

Key words: In situ hybridization, fluorescence, Translocation, genetic, Embryo implantation, Preimplantation diagnosis, Reproductive techniques, assisted