Abstract: Following the development of intracytoplasmic sperm and haploid spermatid injection techniques，the cryopreservation technology of male germ cell, as important part of assisted reproductive technology (ART), has played a key role in the ART treatment for the couples with male infertility. Freezing methods of male germ cells include the programmed cryopreservation and vitrification freezing. Cryoprotectant，usually classified as permeable and impermeable cryoprotective agents，could minimize the cell injuries during the freeze-thaw. Various frozen carriers such as cryoloop and cryoleaf are widely used in cell cryopreservation. In this paper，the freezing method，carrier，cryoprotectants and frozen effect of male germ cell were reviewed.

Key words: Cryopreservation, Semen preservation, Spermatozoa, Spermatids, Sperm injections, intracytoplasmic, Vitrification, Frozen carrier, Cryoprotectant