Abstract: Sperm cryopreservation technique is a basis of assisted reproduction. However，the additional reactive oxygen species （ROS） was produced in the freezing-thawing process，which damaged the physiological structure and function of spermatozoa，and lowered sperm quality and fertility. At present，there were some studies on the protective roles of antioxidants in the sperm cryopreservation. Some natural antioxidants derived from plants are widespread，high efficient and low toxicity. These natural antioxidants can significantly inhibit ROS production，eliminate the sperm damage caused by ROS，and protect sperm activity and genetic stability. In future，these natural antioxidants may be used as additional ingredients in cryoprotective agent for improving sperm cryopreservation.

Key words: Antioxidants, Semen preservation, Cryopreservation, Reactive oxygen species