Abstract: The survival rate of cancer patients has been greatly improved with the advance in diagnosis and treatment technology, however, their fertility is damaged, even lost, by the radiotherapy and chemotherapy. How to effectively preserve fertility is a hot issue in recent years. Trehalose, a disaccharide with special biological properties，is used as a non-permeable cryoprotectant which effectively protect the activities of proteins and other macromolecules in a dry，dehydration，freezing and other harsh conditions. With the research progress，trehalose has been widely used in the medical field，such as the preservation of fertility. Some scholars tried to use it for saving sperm，spermatogonial stem cells，oocytes，embryos，ovarian tissue and testicular tissue, and achieved remarkable results. This review focuses on the biological properties of trehalose，potential mechanism and the research progress of its application in fertility preservation.

Key words: Trehalose, Cryopreservation, Oocytes, Ovary, Spermatozoa