Abstract: Objective: To evaluate the therapeutic efficacy of laparoscopic ovarian puncture for those patients with clomiphene-resistant polycystic ovary syndrome(PCOS). Methods: Seventy-two women with clomifene-resistant PCOS were treated by laparoscopic ovarian puncture from Jan. 2010 to Mar. 2014. Laparoscopic ovarian paracentesis and hydrotubation were jointly performed. The levels of serum FSH, LH, E2, T and PRL were examined, while the ovarian volume and shape were observed before and after the operation. Postoperative ovulation was monitored by B-type ultrasound, and the pregnancy rate was followed up within 2 years after operation. Results: In 72 women with clomifene-resistant PCOS treated by laparoscopic ovarian puncture, ten cases with peritubal and ovarian adhesion were jointly treated by adhesiolysis. Two patients with unilateral tubal blocked were recanulated by the guidewire under hysteroscopy. The levels of LH and T in postoperative 2-month were significantly lower than those before operation (P＜0.05), while the ovarian volume was significantly decreased when compared with that before operation (7.852±3.763 cm3 vs. 11.773±6.553 cm3, t=2.974, P=0.009). The total natural ovulation rate was 61.11% (44/72), and the ovulation rate increased to 86.11% (62/72) when medical therapy was used as well. The cumulated pregnancy rate was 47.2% (34/72) within 24 months after operation. Conclusions: Laparoscopic ovarian puncture is a simple, mini-invasive and safe surgery, which is an alternative treatment for patients with drug-resistant PCOS.

Key words: Polycystic ovary syndrome, Laparoscopes, Clomiphene, Reproductive techniques, assisted