Expressions of AhR and CYP1A1 Protein in Chorionic and Decidual Tissues of Unexplained Embryonic Diapauses and Its Significance

Abstract

Abstract: Objective：To test the expressions of aryl hydrocarbon receptor (AhR) and cytochrome P4501A1 (CYP1A1) protein in the chorionic and decidual tissues of embryonic diapause. Methods：30 cases of early unexplained embryonic diapauses (the study group) and 30 cases of induced abortion in normal early pregnancy (the control group) were selected. The expression levels of AhR and CYP1A1 in the chorionic tissues and decidual tissues were detected by immunohistochemical method. Results：The study group had significantly high expression of AhR protein in the chorionic tissues (0.58±0.03, P＜0.01) and the decidual tissues (0.56±0.03, P＜0.01) when compared with the control group (0.25±0.04, 0.26±0.04). Meanwhile, the study group had significantly high expression of CYP1A1 protein in the chorionic tissues (0.60±0.01, P＜0.01) and the decidual tissues (0.57±0.01, P＜0.01) when compared with the control group (0.22±0.03, 0.24±0.03). Conclusions：The increased expressions of AhR and CYP1A1 protein in the chorionic and decidual tissues may be related to the pathogenesis of embryonic diapauses.

Key words: Embryo loss, Decidua, Chorion, Hydrocarbons, aromatic, Cytochrome P-450 CYP1A1, Embryonic diapauses

WANG Yun-feng，LIAN Li-fen，HOU Hai-yan，LIANG Jing，CHEN Jun，CHEN Ya-qiong，DONG Qu-long. Expressions of AhR and CYP1A1 Protein in Chorionic and Decidual Tissues of Unexplained Embryonic Diapauses and Its Significance[J]. Journal of International Reproductive Health/Family Planning, 2017, 36(6): 451-453.

References

[1] 侯海燕，陈亚琼，王丹等. 天津地区大气颗粒物质与妊娠早期稽留流产的关系[J].国际妇产科学杂志2012, 39（6）：609-612. [2] Perera FP，Rauh V，Tsai W，et al．Effects of transplacental exposure to environmental pollutants on birth outcomes in a multiethnic population［J］．Environ Health Perspect，2003，111( 2) : 201-205． [3]Glinianaia SV,Rankin J,Bell R,et al.Particulate air pollution and fetal health:a systematic review of the epidemiologic evidence[J].Epidemiology,2004,15(1):36-45. [4] Maisonet M,Correa A,Misra D,et al.A review of the literature on the effects of ambient air pollution on fetal growth[J].Environ Res,2004,95(1):106-115. [5] Wu J, Hou H, Ritz B, et al. Exposure to polycyclic aromatic hydrocarbons and missed abortion in early pregnancy in a Chinese population[J].Sci Total Environ ,2010,408(11):2312-2318. [6]张燕萍, 刘旭辉, 任展宏等. 太原市大气污染对妊娠结局的影响[J].环境与健康杂志，2007，24（3）：128-131. [7] Ri t z B, Yu F, Chapa G, et al . Ambieat air pollution and risk of birth defects in southern California [J]. Am J Epidemiol , 2002, 155: 17- 25. [8] 黄莉,王建华.影响胚胎早期发育环境因素的研究进展[J].动物医学进展,2005,25(1):7-10. [9] Dolwick KM, Schmidt JV, Carver LA, et al. Cloning and of a human Ah receptor cDNA. Mol Pharmacol,1993, 44(5):911-7. [10] Jiang YZ, Wang K, Fang R, et al. Expression of aryl hydro-carbon receptor in human placentas and fetal tissues. J Histochem Cytochem, 2010, 58(8):679-85. [11] Stejskalova L, Vecerova L, Perez LM, et al. Aryl hydrocarbon receptor and aryl hydrocarbon nuclear translocator expres-sion in human and rat placentas and transcription activity in human trophoblast cultures. Toxicol Sci, 2011, 123(1):26-36. [12] Peterson RE, Theobald HM, Kimmel GL. Developmental and reproductive toxicity of dioxins and related compounds: cross-species comparisons. CritRev Toxicol,1993, 23(3):283-335. [13] Hernandez-Ochoa I, Karman BN, Flaws JA. The role of the aryl hydrocarbon receptor in the female reproductive system. Biochem Pharmacol, 2009, 77(4):547-59. [14] Li X, Johnson DC, Rozman KK. Reproductive effects of 2, 3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in female rats:ovulation, hormonal regulation, and possible mechanism(s). Toxicol Appl Pharmacol, 1995, 133(2):321-7. [15]Chang TK, Chen J, Yang G, et al. Inhibition of procarcinogen-bioactivating human CYP1A1, CYP1A2 and CYP1B1 enzymes by melatonin[J]. JPinealRes, 2010, 48(1): 55-64. [16]DuffyJE, ZelikoffJT. TheRelationship between noncoplanar PCB-induced immunotoxicity and hepatic CYP1A induction in a fish model[J]. J Immunotoxico,l 2006, 3(1): 39-47. [17]IkutaT, NamikiT, Fuji-iKuriyamaY, etal. AhRprotein trafficking and function in the skin[J]. BiochemPharmaco,l 2009, 77( 4):588-596. [18]KajtaM,WjtowiczAK, Ma kowiakM, et al. Aryl hydrocarbon receptor-mediated apoptosis of neuronal cells: a possible interaction with estrogen receptor signaling[J]. Neuroscience, 2009, 158( 2):811-822. [19]赵娜, 张万起. 芳香烃受体及其介导的二噁英毒性研究进展[J]. 毒理学杂志, 2005, 19( 1): 67-69. [20] 陈莉萍，谭布珍，冯琼等. 滋养层细胞凋亡与稽留流产的关系.[J]2008,23(26): 3712-3716. [21] 古雅丽, 崔豫琳, 邹芳等. 胚胎停止发育患者绒毛中细胞凋亡的表达意义[J] 河南职工医学院学报,2005,17(3),139-140. [22] Vadillo Ortega F, AvilaMA, Hernandez Guerrero C, et al. Apoptosis introphoblast of patients with recurrent spontaneous abortion of unidentified cause[J]. Gynecol Obstet Mex, 2000, 68: 122-131.