Expression of MiR-146a-5p in Decidual Tissue of Unexplained Recurrent Spontaneous Abortion

doi:10.12280/gjszjk.20200669

Abstract

Abstract:

Objective: To study the expression of miR-146a-5p in the decidual tissue of unexplained recurrent miscarriage (URSA), and analyze the possible mechanism of action. Methods: From January to December 2019, ten patients who underwent uterine evacuation due to URSA (URSA group) and ten normal pregnant patients who underwent induced abortion due to no childbirth planning (control group) in Chengdu Xi′nan Gynaecology Hospital were recruited. The decidual tissues were collected. The levels of miR-146a-5p in the decidual tissues were tested. Bioinformatics methods were used to predict the potential target genes of miR-146a-5p and the molecular mechanisms involved. The levels of key target genes of miR-146a-5p in each group were detected. The correlation between miR-146a-5p and the target genes was analyzed.Results: The level of miR-146a-5p in decidual tissue was lower in the URSA group than in the control group (P<0.001). Thirty-five target genes of miR-146a-5p were predicted in at least three databases, among whichIRAK1, CD80, and TRAF6 in the Toll-like receptor (TLR) signaling pathway; and IRAK1, SORT1, and TRAF6 in the neurotrophin (NT) signaling pathway. The levels of IRAK1, CD80, TRAF6 and SORT1, both mRNAs and proteins, in decidual tissue in the URSA group were higher than those in the control group (all P<0.05), and the level of miR-146a-5p was negatively correlated with the expressions of these four molecules (P<0.05).Conclusions: miR-146a-5p in decidual tissue may participate in the pathogenesis of URSA by regulating TLR and NT signaling pathways, which provids new ideas and potential targets for further studies on URSA prevention and therapy.

Key words: Abortion, habitual, Decidua, MicroRNA-146a-5p, Toll-like receptors, Nerve growth factors, Signal transduction

FENG Yu-ting, LUO Shu-hong, LI Lan, SU Qin, CHENG Jing-qiu, YE Hong-xia. Expression of MiR-146a-5p in Decidual Tissue of Unexplained Recurrent Spontaneous Abortion[J]. Journal of International Reproductive Health/Family Planning, 2021, 40(4): 286-293.

Figures/Tables 13

References 28

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基因	引物序列	引物大小（μg/μmol）
IRAK1	上游：GAGAGTGACGAGAGCCTAGG	6 256.13
	下游：CTCGATTCTCCTGCCGTGTC	6 010.94
CD80	上游：GTGGTCACAATGTTTCTGTTGA	6 771.46
	下游：GTTCTTGTACTCGGGCCATATA	6 716.42
TRAF6	上游：GAGACAGGTTTCTTGTGACAAC	6 774.47
	下游：TGGCAACCAAAAGTACTGAATG	6 776.50
SORT1	上游：TCTCAGAGCCGAATGCCGTAGG	6 760.45
	下游：GCCACAATGATGCCTCCAGAATCC	7 266.80

基因	引物序列	引物大小（μg/μmol）
IRAK1	上游：GAGAGTGACGAGAGCCTAGG	6 256.13
	下游：CTCGATTCTCCTGCCGTGTC	6 010.94
CD80	上游：GTGGTCACAATGTTTCTGTTGA	6 771.46
	下游：GTTCTTGTACTCGGGCCATATA	6 716.42
TRAF6	上游：GAGACAGGTTTCTTGTGACAAC	6 774.47
	下游：TGGCAACCAAAAGTACTGAATG	6 776.50
SORT1	上游：TCTCAGAGCCGAATGCCGTAGG	6 760.45
	下游：GCCACAATGATGCCTCCAGAATCC	7 266.80

组别	n	年龄（岁）	胎龄（d）	流产家族史	流产次数（次）	蜕膜组织miR- 146a-5p水平
正常妊娠组	10	28.70±4.79	52.40±5.36	0（0）	0	1.01±0.16
URSA组	10	32.50±3.75	57.10±5.76	1（0.10）	2.30±0.48	0.47±0.17
t或χ²		1.977	1.889	1.053	-	7.452
P		0.064	0.075	0.305	-	<0.001

组别	n	年龄（岁）	胎龄（d）	流产家族史	流产次数（次）	蜕膜组织miR- 146a-5p水平
正常妊娠组	10	28.70±4.79	52.40±5.36	0（0）	0	1.01±0.16
URSA组	10	32.50±3.75	57.10±5.76	1（0.10）	2.30±0.48	0.47±0.17
t或χ²		1.977	1.889	1.053	-	7.452
P		0.064	0.075	0.305	-	<0.001

基因ID	名称	序列
MIMAT0000449	hsa-miR-146a-5p	UGAGAACUGAAUUCCAUGGGUU
MIMAT0000158	mmu-miR-146a-5p	UGAGAACUGAAUUCCAUGGGUU
MIMAT0000852	rno-miR-146a-5p	UGAGAACUGAAUUCCAUGGGUU
MIMAT0001163	gga-miR-146a-5p	UGAGAACUGAAUUCCAUGGGUU
MIMAT0006203	mml-miR-146a-5p	UGAGAACUGAAUUCCAUGGGUU
MIMAT0007230	oan-miR-146a-5p	UGAGAACUGAAUUCCACGGGUU
MIMAT0012746	mdo-miR-146a-5p	UGAGAACUGAAUUCCAUGGGUU
MIMAT0014622	tgu-miR-146a-5p	UGAGAACUGAAUUCCAUGGACU
MIMAT0021775	aca-miR-146a-5p	UGAGAACUGAAUUCCAUAGGC
MIMAT0022635	ola-miR-146a-5p	UGAGAACUGAAUUCCAUAGAUGGUA
MIMAT0022963	ssc-miR-146a-5p	UGAGAACUGAAUUCCAUGGGUU
MIMAT0032371	ssa-miR-146a-5p	UGAGAACUGAAUUCCAUAGAUGG
MIMAT0036567	tch-miR-146a-5p	UGAGAACUGAAUUCCAUGGGUU
MIMAT0037774	cpi-miR-146a-5p	UGAGAACUGAAUUCCAUGUGUUG
MIMAT0038171	ami-miR-146a-5p	UGAGAACUGAAUUCCAUGGGU
MIMAT0038535	cli-miR-146a-5p	UGAGAACUGAAUUCCAUGGGUUG
MIMAT0038961	pbv-miR-146a-5p	UGAGAACUGAAUUCCAUAGGCUU
MIMAT0040181	pal-miR-146a-5p	UGAGAACUGAAUUCCAUGGGUU
MIMAT0046462	xla-miR-146a-5p	UGAGAACUGAAUUCCAUAGGU
MIMAT0047016	cpo-miR-146a-5p	UGAGAACUGAAUUCCAUGGGUUG
MIMAT0047663	dno-miR-146a-5p	UGAGAACUGAAUUCCAUGGGUUU
MIMAT0048253	ocu-miR-146a-5p	UGAGAACUGAAUUCCAUGGGUUG