Abstract: Human embryonic stem cells（hESCs） are the most primitive cells with the potential for self-renewal and differentiation of various cell. The signaling pathways play important roles in regulating cellular morphology and function. Present study confirmed that the Wnt signaling pathway, as one of the key factors of differentiation, played an important role in regulating the development of female reproductive system. This pathway plays same key role during the differentiation of endometrial-like cells from embryonic stem cell. This review is about the Wnt signaling pathway in hESCs differentiation to endometrial cells，and the future research.

Key words: Embryonic stem cells, Wnt proteins, Endometrium, Cell differentiation, Gene regulatory networks