富血小板血浆改善卵巢储备功能的相关机制

doi:10.12280/gjszjk.20230087

摘要/Abstract

摘要：

富血小板血浆（platelet-rich plasma，PRP）是从全血离心提取的浓缩物，其血小板浓度是循环血清血小板浓度的7倍，富含大量生长因子和细胞因子，其中一些因子可通过诱导趋化性、细胞迁移和分化来促进组织愈合和再生。卵巢储备功能是指女性卵巢内存留卵子的质量和数量，是衡量女性生育潜能的重要标志。基于目前国内外基础研究和临床试验，探讨PRP通过促进卵巢血管的生成、减少颗粒细胞凋亡和卵泡的闭锁、促进卵丘扩张、增加线粒体活性途径及促进卵母细胞发育对改善卵巢储备功能的作用，以期为PRP对卵巢储备功能降低的女性行辅助生殖助孕提供理论依据。

关键词: 富血小板血浆, 卵巢储备功能, 不育，女（雌）性, 卵母细胞

Abstract:

The platelet-rich plasma (PRP) is a concentrate extracted from whole blood centrifugation at a platelet concentration of up to 7 times that of circulating serum, being rich in growth factors and cytokines. Some of factors promote tissue healing and regeneration by inducing chemotaxis, cell migration and differentiation. Ovarian reserve refers to the quality and quantity of eggs retained in ovaries, which is an important indicator of female fertility potential. Based on the current basic research and clinical trials at home and abroad, it is proposed that PRP can promote ovarian vascular production, reduce apoptosis of granule cells and atresia of follicles, promote cumulus dilation, increase mitochondrial activity pathway and promote oocyte development. This review may provide a theoretical basis for the clinical practice of PRP in the assisted reproduction of those women with the reduced ovarian reserve.

Key words: Platelet-rich plasma, Ovarian reserve, Infertility, female, Oocytes

柳絮, 杨爱军, 李泽武, 石城, 刘利君, 孔潇丽, 王靖雯. 富血小板血浆改善卵巢储备功能的相关机制[J]. 国际生殖健康/计划生育杂志, 2023, 42(4): 329-333.

LIU Xu, YANG Ai-jun, LI Ze-wu, SHI Cheng, LIU Li-jun, KONG Xiao-li, WANG Jing-wen. The Mechanism of Platelet-Rich Plasma on Improving Ovarian Reserve[J]. Journal of International Reproductive Health/Family Planning, 2023, 42(4): 329-333.

参考文献 29

[1]	Johnson J, Canning J, Kaneko T, et al. Germline stem cells and follicular renewal in the postnatal mammalian ovary[J]. Nature, 2004, 428(6979):145-150. doi: 10.1038/nature02316. doi: 10.1038/nature02316
[2]	Chamata ES, Bartlett EL, Weir D, et al. Platelet-Rich Plasma: Evolving Role in Plastic Surgery[J]. Plast Reconstr Surg, 2021, 147(1):219-230. doi: 10.1097/PRS.0000000000007509. doi: 10.1097/PRS.0000000000007509 pmid: 33370070
[3]	Mercuri SR, Vollono L, Paolino G. The Usefulness of Platelet-Rich Plasma (PRP) for the Treatment of Vitiligo: State of the Art and Review[J]. Drug Des Devel Ther, 2020, 14:1749-1755. doi: 10.2147/DDDT.S239912. doi: 10.2147/DDDT.S239912 URL
[4]	Sdeek M, Sabry D, El-Sdeek H, et al. Intra-articular injection of Platelet rich plasma versus Hyaluronic acid for moderate knee osteoarthritis. A prospective, double-blind randomized controlled trial on 189 patients with follow-up for three years[J]. Acta Orthop Belg, 2021, 87(4):729-734. doi: 10.52628/87.4.18. doi: 10.52628/87.4.18 pmid: 35172440
[5]	Shao S, Pan R, Chen Y. Autologous Platelet-Rich Plasma for Diabetic Foot Ulcer[J]. Trends Endocrinol Metab, 2020, 31(12):885-890. doi: 10.1016/j.tem.2020.10.003. doi: 10.1016/j.tem.2020.10.003 URL
[6]	Chang Y, Li J, Wei LN, et al. Autologous platelet-rich plasma infusion improves clinical pregnancy rate in frozen embryo transfer cycles for women with thin endometrium[J]. Medicine(Baltimore), 2019, 98(3):e14062. doi: 10.1097/MD.0000000000014062. doi: 10.1097/MD.0000000000014062
[7]	沈真如, 金纯纯, 席瑾, 等. 基于DLL4/Notch1信号通路的麦粒灸对薄型子宫内膜大鼠血管生成的影响[J]. 中国中医药信息杂志, 2021, 28(9):73-78. doi: 10.19879/j.cnki.1005-5304.202012526. doi: 10.19879/j.cnki.1005-5304.202012526
[8]	Zhou Y, Shen H, Wu Y, et al. Platelet-Rich Plasma Therapy Enhances the Beneficial Effect of Bone Marrow Stem Cell Transplant on Endometrial Regeneration[J]. Front Cell Dev Biol, 2020, 8:52. doi: 10.3389/fcell.2020.00052. doi: 10.3389/fcell.2020.00052 pmid: 32154246
[9]	Ahmadian S, Sheshpari S, Pazhang M, et al. Intra-ovarian injection of platelet-rich plasma into ovarian tissue promoted rejuvenation in the rat model of premature ovarian insufficiency and restored ovulation rate via angiogenesis modulation[J]. Reprod Biol Endocrinol, 2020, 18(1):78. doi: 10.1186/s12958-020-00638-4. doi: 10.1186/s12958-020-00638-4
[10]	Melincovici CS, Boşca AB, Şuşman S, et al. Vascular endothelial growth factor (VEGF) - key factor in normal and pathological angiogenesis[J]. Rom J Morphol Embryol, 2018, 59(2):455-467.
[11]	Akwii RG, Sajib MS, Zahra FT, et al. Role of Angiopoietin-2 in Vascular Physiology and Pathophysiology[J]. Cells, 2019, 8(5):471. doi: 10.3390/cells8050471. doi: 10.3390/cells8050471 URL
[12]	Huang Q, Liu B, Jiang R, et al. G-CSF-mobilized peripheral blood mononuclear cells combined with platelet-rich plasma accelerate restoration of ovarian function in cyclophosphamide-induced POI rats?[J]. Biol Reprod, 2019, 101(1):91-101. doi: 10.1093/biolre/ioz077. doi: 10.1093/biolre/ioz077 URL
[13]	Tamanini C, De Ambrogi M. Angiogenesis in developing follicle and corpus luteum[J]. Reprod Domest Anim, 2004, 39(4):206-216. doi: 10.1111/j.1439-0531.2004.00505.x. doi: 10.1111/j.1439-0531.2004.00505.x pmid: 15225273
[14]	Reynolds LP, Grazul-Bilska AT, Redmer DA. Angiogenesis in the corpus luteum[J]. Endocrine, 2000, 12(1):1-9. doi: 10.1385/ENDO:12:1:1. doi: 10.1385/ENDO:12:1:1 pmid: 10855683
[15]	Dolmans MM, Cacciottola L, Amorim CA, et al. Translational research aiming to improve survival of ovarian tissue transplants using adipose tissue-derived stem cells[J]. Acta Obstet Gynecol Scand, 2019, 98(5):665-671. doi: 10.1111/aogs.13610. doi: 10.1111/aogs.13610 URL
[16]	Siemerink MJ, Klaassen I, Vogels IM, et al. CD34 marks angiogenic tip cells in human vascular endothelial cell cultures[J]. Angiogenesis, 2012, 15(1):151-163. doi: 10.1007/s10456-011-9251-z. doi: 10.1007/s10456-011-9251-z pmid: 22249946
[17]	Callejo J, Salvador C, González-Nuñez S, et al. Live birth in a woman without ovaries after autograft of frozen-thawed ovarian tissue combined with growth factors[J]. J Ovarian Res, 2013, 6(1):33. doi: 10.1186/1757-2215-6-33. doi: 10.1186/1757-2215-6-33 pmid: 23647552
[18]	朱文倩, 张慧敏, 李磊, 等. 激活的富血小板血浆对卵巢储备功能降低患者卵丘颗粒细胞凋亡和增殖的影响[J]. 生殖医学杂志, 2022, 31(5):652-660. doi: 10.3969/j.issn.1004-3845.2022.05.014. doi: 10.3969/j.issn.1004-3845.2022.05.014
[19]	Vural B, Duruksu G, Vural F, et al. Effects of VEGF + Mesenchymal Stem Cells and Platelet-Rich Plasma on Inbred Rat Ovarian Functions in Cyclophosphamide-Induced Premature Ovarian Insufficiency Model[J]. Stem Cell Rev Rep, 2019, 15(4):558-573. doi: 10.1007/s12015-019-09892-5. doi: 10.1007/s12015-019-09892-5
[20]	Huang Z, Wells D. The human oocyte and cumulus cells relationship: new insights from the cumulus cell transcriptome[J]. Mol Hum Reprod, 2010, 16(10):715-725. doi: 10.1093/molehr/gaq031. doi: 10.1093/molehr/gaq031 pmid: 20435609
[21]	Moulavi F, Akram RT, Khorshid Sokhangouy S, et al. Platelet rich plasma efficiently substitutes the beneficial effects of serum during in vitro oocyte maturation and helps maintain the mitochondrial activity of maturing oocytes[J]. Growth Factors, 2020, 38(3/4):152-166. doi: 10.1080/08977194.2021.1900168. doi: 10.1080/08977194.2021.1900168 URL
[22]	Labarta E, de Los Santos MJ, Escribá MJ, et al. Mitochondria as a tool for oocyte rejuvenation[J]. Fertil Steril, 2019, 111(2):219-226. doi: 10.1016/j.fertnstert.2018.10.036. doi: S0015-0282(18)32162-9 pmid: 30611551
[23]	Pazoki H, Eimani H, Farokhi F, et al. The effects of platelet lysate on maturation, fertilization and embryo development of NMRI mouse oocytes at germinal vesicle stage[J]. Reprod Med Biol, 2016, 15(2):115-120. doi: 10.1007/s12522-015-0220-x. doi: 10.1007/s12522-015-0220-x pmid: 29259427
[24]	Sills ES, Rickers NS, Li X, et al. First data on in vitro fertilization and blastocyst formation after intraovarian injection of calcium gluconate-activated autologous platelet rich plasma[J]. Gynecol Endocrinol, 2018, 34(9):756-760. doi: 10.1080/09513590.2018.1445219. doi: 10.1080/09513590.2018.1445219 pmid: 29486615
[25]	Melo P, Navarro C, Jones C, et al. The use of autologous platelet-rich plasma (PRP) versus no intervention in women with low ovarian reserve undergoing fertility treatment: a non-randomized interventional study[J]. J Assist Reprod Genet, 2020, 37(4):855-863. doi: 10.1007/s10815-020-01710-z. doi: 10.1007/s10815-020-01710-z
[26]	Barad DH, Albertini DF, Molinari E, et al. Preliminary report of intraovarian injections of autologous platelet-rich plasma (PRP) in extremely poor prognosis patients with only oocyte donation as alternative: a prospective cohort study[J]. Hum Reprod Open, 2022, 2022(3): hoac027. doi: 10.1093/hropen/hoac027. doi: 10.1093/hropen/hoac027
[27]	Cakiroglu Y, Saltik A, Yuceturk A, et al. Effects of intraovarian injection of autologous platelet rich plasma on ovarian reserve and IVF outcome parameters in women with primary ovarian insufficiency[J]. Aging (Albany NY), 2020, 12(11):10211-10222. doi: 10.18632/aging.103403. doi: 10.18632/aging.103403
[28]	Panda SR, Sachan S, Hota S. A Systematic Review Evaluating the Efficacy of Intra-Ovarian Infusion of Autologous Platelet-Rich Plasma in Patients With Poor Ovarian Reserve or Ovarian Insufficiency[J]. Cureus, 2020, 12(12):e12037. doi: 10.7759/cureus.12037. doi: 10.7759/cureus.12037
[29]	Cakiroglu Y, Yuceturk A, Karaosmanoglu O, et al. Ovarian reserve parameters and IVF outcomes in 510 women with poor ovarian response (POR) treated with intraovarian injection of autologous platelet rich plasma (PRP)[J]. Aging (Albany NY), 2022, 14(6):2513-2523. doi: 10.18632/aging.203972. doi: 10.18632/aging.203972